Anti-Hu CD4 Purified

Anti-Hu CD4 Purified
Regulatory status
RUO
Antigen
CD4
Clone
EM4
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested)
Variant
0.1 mg
11-816-C100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody EM4 recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T-cells) and also on monocytes, tissue macrophages and granulocytes. This antibody does not block Leu3a and OKT4 binding, and blocks HIV-1 infection in cell to cell system. Very strong flow cytometry staining, brighter than Leu3a, OKT4 and other.
Application
IP, WB, FC (QC tested)
Reactivity
Human
Immunogen
Normal human blood lymphocytes
Other names
T4/Leu-3, L3T4
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-816 FC
Flow cytometry analysis (surface staining) of human buffy coat cells with anti-CD4 (EM4) purified / GAM-APC.

General references:

Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53(1):33-40.
PubMed

Product specific references:

Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
PubMed
Variant
0.1 mg
11-816-C100
In stock
Variant
0.1 mg
11-261-C100
In stock