Anti-Hu CD38 FITC

Anti-Hu CD38 FITC
Regulatory status
RUO
Antigen
CD38
Clone
HB7
Format
FITC
Reactivity
Human
Excitation laser
blue (488 nm)
Variant
100 tests
1F-915-T100
In stock
198.00 USD
Variant
100 tests
1F-915-T100
In stock
198.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody HB7 (HB-7) recognizes an extracellular epitope within amino acids 273-285 of human CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab.
Workshop
HCDM III
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
BJAB cell line
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ADPRC1, cADPr hydrolase 1, T10, NAD(+) nucleosidase, ADP-ribosyl cyclase 1
Antigen description
CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
Entrez Gene ID 952
UniProt ID P28907
1F-915_FC_CDMaps_Profil značení
Anti-human CD38 FITC antibody (clone HB7) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD38 FITC antibody (clone HB7) was used in concentration 0.5 µg/ml in stained blood sample (2 x 106 cells).
1F-915_FC_CDMaps_Dot plot
Staining pattern of Anti-human CD38 FITC antibody (clone HB7) in dot-plot fluorescence visualization (B cell gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD38 FITC antibody (clone HB7) in concentration 0.5 µg/ml in stained stained blood sample (2 x 106 cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) was used in concentration 2 µg/ml, respectively.
1F-915_FC_CDMaps_Dot plot2
Staining pattern of Anti-human CD38 FITC antibody (clone HB7) in dot-plot fluorescence visualization (B cell gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD38 FITC antibody (clone HB7) in concentration 0.5 µg/ml in stained stained blood sample (2 x 106 cells) and Mouse monoclonal anti-human CD138 APC antibody (clone MI15) in concentration 2 µg/ml, respectively.
1F-915_FC_CDMaps_Histogram
Reactivity of Anti-human CD38 FITC antibody (clone HB7) on human peripheral leukocytes

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Suspension of blood leukocytes (2 x 106 cells) was added to the mixture of CD38 FITC antibody (clone HB7, 0.5 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

General references:

Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP-ribose-CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase-catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1;358(Pt 2):399-406.
PubMed
Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro-B leukemic cell line by a tyrosine kinase-dependent but ADP-ribosyl cyclase- and NAD glycohydrolase-independent mechanism. Int Immunol. 2006 Jul;18(7):1029-42.
PubMed
Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE. Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp-ribose, the major product generated by the CD38 enzyme reaction. J Immunol. 2007 Dec 1;179(11):7827-39.
PubMed

Product specific references:

Goldmacher VS, Bourret LA, Levine BA, Rasmussen RA, Pourshadi M, Lambert JM, Anderson KC: Anti-CD38-blocked ricin: an immunotoxin for the treatment of multiple myeloma. Blood 1994 Nov 1;84(9):3017-25.
PubMed
Zhao YJ, Zhu WJ, Wang XW, Zhang LH, Lee HC: Determinants of the membrane orientation of a calcium signaling enzyme CD38. Biochim Biophys Acta 2015 Sep;1853(9):2095-103.
PubMed
Berthelier V, Laboureau J, Boulla G, Schuber F, Deterre P: Probing ligand-induced conformational changes of human CD38. Eur J Biochem. 2000 May;267(10):3056-64.
PubMed
Li T, Qi S, Unger M, Hou YN, Deng QW, Liu J, Lam CM, Wang XW, Xin D, Zhang P, Koch-Nolte F, Hao Q, Zhang H, Lee HC, Zhao YJ: Immuno-targeting the multifunctional CD38 using nanobody. Sci Rep. 2016 Jun 2;6:27055.
PubMed
Ghose J, Viola D, Terrazas C, Caserta E, Troadec E, Khalife J, Gunes EG, Sanchez J, McDonald T, Marcucci G, Kaur B, Rosenzweig M, Keats J, Rosen S, Krishnan A, Satoskar AR, Hofmeister CC, Pichiorri F: Daratumumab induces CD38 internalization and impairs myeloma cell adhesion. Oncoimmunology. 2018 Jul 23;7(10):e1486948.
PubMed
Ferrero E, Orciani M, Vacca P, Ortolan E, Crovella S, Titti F, Saccucci F, Malavasi F: Characterization and phylogenetic epitope mapping of CD38 ADPR cyclase in the cynomolgus macaque. BMC Immunol. 2004 Sep 21;5:21.
PubMed
Ratuszny D, Skripuletz T, Wegner F, Groß M, Falk C, Jacobs R, Ruschulte H, Stangel M, Sühs KW: Case report: Daratumumab in a patient with severe refractory anti-NMDA receptor encephalitis. Front Neurol. 2020 Dec 22;11:602102.
PubMed
Variant
100 tests
1F-915-T100
In stock
198.00 USD
Variant
100 tests
1F-915-T100
In stock
198.00 USD

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