Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD371 Purified

Anti-Hu CD371 Purified

Regulatory status

RUO

Antigen

CD371

Clone

50C1

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-953-C100

In stock

158.40 USD

Variant

0.1 mg

11-953-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody 50C1 recognizes an extracellular epitope of CD371, a type II transmembrane glycoprotein expressed above all on monocytes, macrophages, granulocytes and dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

293T cells expressing human CD371-Flag

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLEC12A, CLL1, MICL, DCAL-2

Antigen description

CD371, also known as CLEC12A, is a 30 kDa type II transmembrane glycoprotein of the C-type lectin family. Several alternatively spliced isoforms have been described. Using ITIM in its cytoplasmic part, this receptor serves as a negative regulator of CD371-positive cells, mainly granulocytes and monocytes, and is involved in dendritic cell maturation. It is one of markers of myeloid cells, including acute myeloid leukemia.

Entrez Gene ID 160364

UniProt ID Q5QGZ9

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD371 (50C1) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD371 (50C1) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Product specific references:

Lahoud MH, Proietto AI, Ahmet F, Kitsoulis S, Eidsmo L, Wu L, Sathe P, Pietersz S, Chang HW, Walker ID, Maraskovsky E, Braley H, Lew AM, Wright MD, Heath WR, Shortman K, Caminschi I: The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses. J Immunol. 2009 Jun 15;182(12):7587-94.
PubMed

Coustan-Smith E, Song G, Shurtleff S, Yeoh AE, Chng WJ, Chen SP, Rubnitz JE, Pui CH, Downing JR, Campana D: Universal monitoring of minimal residual disease in acute myeloid leukemia. JCI Insight 2018 May 3;3(9):e98561.
PubMed

Shameli A, Dharmani-Khan P, Luider J, Auer I, Shabani-Rad MT: Exploring blast composition in myelodysplastic syndromes and myelodysplastic/myeloproliferative neoplasms: CD45RA and CD371 improve diagnostic value of flow cytometry through assessment of myeloblast heterogeneity and stem cell aberrancy. Cytometry B Clin Cytom. 2021 Sep;100(5):574-589.
PubMed