Anti-Hu CD371 PE

Anti-Hu CD371 PE
Regulatory status
RUO
Antigen
CD371
Clone
50C1
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-953-T100
In stock
242.00 USD
Variant
100 tests
1P-953-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody 50C1 recognizes an extracellular epitope of CD371, a type II transmembrane glycoprotein expressed above all on monocytes, macrophages, granulocytes and dendritic cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
293T cells expressing human CD371-Flag
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CLEC12A, CLL1, MICL, DCAL-2
Antigen description
CD371, also known as CLEC12A, is a 30 kDa type II transmembrane glycoprotein of the C-type lectin family. Several alternatively spliced isoforms have been described. Using ITIM in its cytoplasmic part, this receptor serves as a negative regulator of CD371-positive cells, mainly granulocytes and monocytes, and is involved in dendritic cell maturation. It is one of markers of myeloid cells, including acute myeloid leukemia.
Entrez Gene ID 160364
UniProt ID Q5QGZ9
1P-953_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD371 (50C1) PE antibody (concentration in sample 0.56 μg/ml).
1P-953_FC_Histogram
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD371 (50C1) PE antibody (concentration in sample 0.56 μg/ml).

Product specific references:

Lahoud MH, Proietto AI, Ahmet F, Kitsoulis S, Eidsmo L, Wu L, Sathe P, Pietersz S, Chang HW, Walker ID, Maraskovsky E, Braley H, Lew AM, Wright MD, Heath WR, Shortman K, Caminschi I: The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses. J Immunol. 2009 Jun 15;182(12):7587-94.
PubMed
Coustan-Smith E, Song G, Shurtleff S, Yeoh AE, Chng WJ, Chen SP, Rubnitz JE, Pui CH, Downing JR, Campana D: Universal monitoring of minimal residual disease in acute myeloid leukemia. JCI Insight 2018 May 3;3(9):e98561.
PubMed
Shameli A, Dharmani-Khan P, Luider J, Auer I, Shabani-Rad MT: Exploring blast composition in myelodysplastic syndromes and myelodysplastic/myeloproliferative neoplasms: CD45RA and CD371 improve diagnostic value of flow cytometry through assessment of myeloblast heterogeneity and stem cell aberrancy. Cytometry B Clin Cytom. 2021 Sep;100(5):574-589.
PubMed
Variant
100 tests
1P-953-T100
In stock
242.00 USD
Variant
100 tests
1P-953-T100
In stock
242.00 USD

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