Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD37 Alexa Fluor<sup>®</sup> 647

Anti-Hu CD37 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD37

Clone

MB-1

Format

Alexa Fluor^® 647

Reactivity

Human

Application

FC (QC tested), IHC-F

Excitation laser

red (633 nm)

Variant

100 tests

A6-923-T100

In stock

242.00 USD

Variant

100 tests

A6-923-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody MB-1 recognizes an extracellular epitope of CD37, a tetraspanin family transmembrane glycoprotein.

Application

FC (QC tested), IHC-F

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

lymphomatous lymph node cells

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

GP52-40, tetraspanin 26

Antigen description

CD37 is a 40-64 kDa tetraspanin family glycoprotein, which forms complexes in the B cell membrane with MHC class II, CD53, CD81, and CD82. It is expressed highly on mature B cells and neoplastic B cells, but it is lost on plasma cells, as well as on pro-B cells. Lower expression was detected on monocytes, macrophages, and dendritic cells.

Entrez Gene ID 951

UniProt ID P11049

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD37 (MB-1) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD37 (MB-1) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD37 positive CD19 positive B cells (red-filled) from CD37 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD37 (MB-1) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Link MP, Bindl J, Meeker TC, Carswell C, Doss CA, Warnke RA, Levy R: A unique antigen on mature B cells defined by a monoclonal antibody. J Immunol. 1986 Nov 1;137(9):3013-8.
PubMed

Press OW, Eary JF, Badger CC, Martin PJ, Appelbaum FR, Levy R, Miller R, Brown S, Nelp WB, Krohn KA: Treatment of refractory non-Hodgkin's lymphoma with radiolabeled MB-1 (anti-CD37) antibody. J Clin Oncol. 1989 Aug;7(8):1027-38.
PubMed