Anti-Hu CD369 Purified

Anti-Hu CD369 Purified
Regulatory status
RUO
Antigen
CD369
Clone
15E2
Format
Purified
Reactivity
Human
Application
FC (QC tested), IHC(F)
Variant
0.1 mg
11-874-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.
Workshop
HLDA X
Application
FC (QC tested), IHC(F)
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Human
Immunogen
Extracellular part of human CD369 with hIgG FC tag
Other names
Dectin-1, CLEC7A, BGR, CANDF4, SCARE2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-874_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD369 (15E2) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).
11-874_FC_Histogram
Separation of monocytes stained anti-human CD369 (15E2) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Ni L, Gayet I, Zurawski S, Duluc D, Flamar AL, Li XH, O'Bar A, Clayton S, Palucka AK, G Zurawski, Banchereau J, Oh SK: Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010 Sep 15;185(6):3504-13
PubMed
Fischer M, Müller JP, Spies-Weisshart B, Gräfe C, Kurzai O, Hünniger K, Hochhaus A, Scholl S, Schnetzke U. Isoform localization of dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 2017 May;47(5):848-859
PubMed
Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li XH, Hori T, Zurawski S, Liu YJ, Zurawski G, Oh S: Opposing roles of dectin-1 expressed on human plasmacytoid dendritic cells and myeloid dendritic cells in Th2 polarization. J Immunol. 2015 Aug 15;195(4):1723-31
PubMed
Variant
0.1 mg
11-874-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD