Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD369 Purified

Anti-Hu CD369 Purified

Regulatory status

RUO

Antigen

CD369

Clone

15E2

Format

Purified

Reactivity

Human

Application

FC (QC tested), IHC(F)

Variant

0.1 mg

11-874-C100

In stock

154.00 USD

Variant

0.1 mg

11-874-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.

Workshop

HLDA X

Application

FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Reactivity

Human

Immunogen

Extracellular part of human CD369 with hIgG FC tag

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Dectin-1, CLEC7A, BGR, CANDF4, SCARE2

Antigen description

CD369 (dectin-1, beta-glucan receptor) is a 33 kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.

Entrez Gene ID 64581

UniProt ID Q9BXN2

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD369 (15E2) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Separation of monocytes stained anti-human CD369 (15E2) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Ni L, Gayet I, Zurawski S, Duluc D, Flamar AL, Li XH, O'Bar A, Clayton S, Palucka AK, G Zurawski, Banchereau J, Oh SK: Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010 Sep 15;185(6):3504-13
PubMed

Fischer M, Müller JP, Spies-Weisshart B, Gräfe C, Kurzai O, Hünniger K, Hochhaus A, Scholl S, Schnetzke U. Isoform localization of dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 2017 May;47(5):848-859
PubMed

Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li XH, Hori T, Zurawski S, Liu YJ, Zurawski G, Oh S: Opposing roles of dectin-1 expressed on human plasmacytoid dendritic cells and myeloid dendritic cells in Th2 polarization. J Immunol. 2015 Aug 15;195(4):1723-31
PubMed