Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD368 APC

Anti-Hu CD368 APC

Regulatory status

RUO

Antigen

CD368

Clone

9B9

Format

APC

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-931-T100

In stock

242.00 USD

Variant

100 tests

1A-931-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 9B9 recognizes an extracellular epitope of CD368, a type II transmembrane protein of C lectin family, expressed mainly on monocytes and neutrophils.

Workshop

HLDA X

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

CD368 ectodomain fused with human Fc

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLEC6, CLEC4D, dectin 3, MCL, MPCL

Antigen description

CD368 is an approximately 25-30 kDa C-type lectin, which serves as one of pattern recognition receptors of the innate immune system. It recognizes pathogen-associated molecular patterns of bacteria and fungi, such as alpha-mannans or trehalose 6,6´-dimycolate, and transfers the signal downstream using associated Fc receptor gamma chain. CD368 is expressed on neutrophils, monocytes, and on some populations of blood dendritic cells, but it decreases during differentiation of monocytes into dendritic cells or macrophages. Increased expression can be induced by IFN-gamma, TNF-alpha, IL-6, and IL-10. CD368 triggering leads to driving of antigen-presenting cells maturation and to promotion of T cell differentiation into Th1 and Th17.

Entrez Gene ID 338339

UniProt ID Q8WXI8

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD368 (9B9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD368 (9B9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Damasceno D, Andrés MP, van den Bossche WB, Flores-Montero J, de Bruin S, Teodosio C, van Dongen JJ, Orfao A, Almeida J: Expression profile of novel cell surface molecules on different subsets of human peripheral blood antigen-presenting cells. Clin Transl Immunology 2016 Sep 16;5(9):e100.
PubMed

Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ: Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 workshop in Wollongong, Australia. Clin Transl Immunology 2015 Nov 13;4(11):e50.
PubMed

Clark G, Stockinger H, Balderas R, van Zelm MC, Zola H, Hart D, Engel P: Nomenclature of CD molecules from the Tenth human leucocyte differentiation antigen workshop. Clin Transl Immunology 2016 Jan 22;5(1):e57.
PubMed