Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD367 PE

Anti-Hu CD367 PE

Regulatory status

RUO

Antigen

CD367

Clone

9E8

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-930-T100

In stock

242.00 USD

Variant

100 tests

1P-930-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 9E8 recognizes an extracellular epitope of human CD367, a type II transmembrane protein of C-lectin family, expressed mainly on antigen presenting cells.

Workshop

HLDA X

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

CD367 ectodomain fused with human Fc

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DCIR, CLEC4A, LLIR

Antigen description

CD367 is an approximately 20-28 kDa C-type lectin with immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic part. CD367 binds in calcium-dependent manner to mannose, fucose, and weakly also to N-acetylglucosamine. It is expressed on dendritic cells, macrophages, monocytes, B cells, and neutrophils. In rheumatoid arthitis patients CD367 is expressed also on CD4+ T cells. After ligand-mediated triggering, it is internalized by clathrin-dependent endocytosis and contributes to the antigen presentation to CD8+ T cells. It may also be involved in modulation of the antigen presenting cell response.

Entrez Gene ID 50856

UniProt ID Q9UMR7

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Klechevsky E, Flamar AL, Cao Y, Blanck JP, Liu M, O'Bar A, Agouna-Deciat O, Klucar P, Thompson-Snipes L, Zurawski S, Reiter Y, Palucka AK, Zurawski G, Banchereau J: Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR. Blood 2010 Sep 9;116(10):1685-97.
PubMed

Damasceno D, Andrés MP, van den Bossche WB, Flores-Montero J, de Bruin S, Teodosio C, van Dongen JJ, Orfao A, Almeida J: Expression profile of novel cell surface molecules on different subsets of human peripheral blood antigen-presenting cells. Clin Transl Immunology 2016 Sep 16;5(9):e100.
PubMed

Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ: Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 workshop in Wollongong, Australia. Clin Transl Immunology 2015 Nov 13;4(11):e50.
PubMed