Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD361 Purified

Anti-Hu CD361 Purified

Regulatory status

RUO

Antigen

CD361

Clone

MEM-216

Format

Purified

Reactivity

Human

Application

IP, FC (QC tested)

Variant

0.1 mg

11-643-C100

In stock

143.00 USD

0.025 mg

11-643-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-643-C100

In stock

143.00 USD

0.025 mg

11-643-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody MEM-216 recognizes an extracellular epitope of CD361 / EVI2B, almost uncharacterized type I transmembrane protein with broad leukocyte expression, mostly in myeloid and B cells.

Workshop

HLDA IX: WS Code 263

Application

IP, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml, Positive control, Positive control: Raji, Daudi, HL-60 cells, peripheral blood lymphcocytes (strongly positive on CD19+ cells), negative control: Jurkat, U-937 cells.

Reactivity

Human

Immunogen

Raji cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

EVI2B, EVDB

Antigen description

CD361, also known as EVI2B (ecotropic viral integration site 2B) or EVDB, is a poorly characterized type I transmembrane protein, expressed from one of three genes embedded in intron 27b of the neurofibromatosis type 1 (NF1) gene. The DNA strand that is transcribed to produce CD361 is the complementary one to the strand encoding NF1. Murine homolog to human CD361 is associated with ecotropic viral insertions, which have been implicated in the expression of murine myeloid leukemias. CD361 has been also reported to be involved in melanocyte and keratinocyte differentiation. However, it is expressed mainly in peripheral blood and bone marrow.

Entrez Gene ID 2124

UniProt ID P34910

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD361 (MEM-216) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD361 (MEM-216) purified antibody (concentration in sample 4 μg/ml, GAM APC).

General references:

Vyskocil D, Cawthon R, O'Connell P, Xu GF, Stevens J, Culver M, Carey J, White R: The gene encoding the oligodendrocyte-myelin glycoprotein is embedded within the neurofibromatosis type 1 gene. Mol Cell Biol. 1991 Feb;11(2):906-12.
PubMed

Kaufmann D, Gruener S, Braun F, Stark M, Griesser J, Hoffmeyer S, Bartelt B: EVI2B, a gene lying in an intron of the neurofibromatosis type 1 (NF1) gene, is as the NF1 gene involved in differentiation of melanocytes and keratinocytes and is overexpressed in cells derived from NF1 neurofibromas. DNA Cell Biol. 1999 May;18(5):345-56.
PubMed

Product specific references:

Rudolf-Oliveira RCM, Auat M, Cardoso CC, Santos-Pirath IM, Lange BG, Pires-Silva J, Moraes ACR, Dametto GC, Pirolli MM, Colombo MDHP, Santos-Silva MC: Determination of normal expression patterns of CD86, CD210a, CD261, CD262, CD264, CD358, and CD361 in peripheral blood and bone marrow cells by flow cytometry.
PubMed

Mouloungui E, Zver T, Roux C, Amiot C: A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications. J Ovarian Res. 2018 Jan 5;11(1):4. doi: 10.1186/s13048-017-0376-6.
PubMed