Anti-Hu CD36 APC

Anti-Hu CD36 APC
Regulatory status
RUO
Antigen
CD36
Clone
CB38
Format
APC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
1A-648-T100
In stock

25 tests
1A-648-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgM kappa
Specificity
The mouse monoclonal antibody CB38 (NL07) recognizes CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
Workshop
HLDA IV: WS Code P106
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
living human myeloid cells
Other names
GPIIIb, Platelet glycoprotein 4, Platelet glycoprotein IV, GPIV, Glycoprotein IIIb, GPIIIB, PAS IV, PAS-4, Platelet collagen receptor, Fatty acid translocase, FAT, Thrombospondin receptor, PASIV
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1A-648 FC
Flow cytometry analysis (surface staining) of CD36 in human peripheral blood with anti-CD36 (CB38) APC.

General references:

Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. FASEB J. 2007 Dec 27
PubMed
van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. Biochim Biophys Acta. 2007 Dec 15
PubMed
Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. J Immunol. 2008 Jan 1;180(1):230-7.
PubMed

Product specific references:

Kapinsky M, Torzewski M, Büchler C, Duong CQ, Rothe G, Schmitz G: Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger-receptor CD36. Arterioscler Thromb Vasc Biol. 2001 Jun;21(6):1004-10.
PubMed
McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH: Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry. Blood. 2001 Oct 15;98(8):2498-507.
PubMed
Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun;13(6):704-7.
PubMed
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F: Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell Immunol. 1991 Oct 15;137(2):487-500.
PubMed
Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr Stud Hematol Blood Transfus. 1991;(58):182-6.
PubMed
Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F: Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood. 1993 Dec 15;82(12):3637-47.
PubMed
Fadok VA, Warner ML, Bratton DL, Henson PM: CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol. 1998 Dec 1;161(11):6250-7.
PubMed
Matasić R, Dietz AB, Vuk-Pavlović S: Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells. J Leukoc Biol. 1999 Dec;66(6):909-14.
PubMed
Hoffmann PR, deCathelineau AM, Ogden CA, Leverrier Y, Bratton DL, Daleke DL, Ridley AJ, Fadok VA, Henson PM: Phosphatidylserine (PS) induces PS receptor-mediated macropinocytosis and promotes clearance of apoptotic cells. J Cell Biol. 2001 Nov 12;155(4):649-59.
PubMed
Variant
100 tests
1A-648-T100
In stock

25 tests
1A-648-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock