Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD36 Alexa Fluor<sup>®</sup> 700

Anti-Hu CD36 Alexa Fluor^® 700

Regulatory status

RUO

Antigen

CD36

Clone

TR9

Format

Alexa Fluor^® 700

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

A7-451-T100

In stock

242.00 USD

Variant

100 tests

A7-451-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody TR9 reacts with an extracellular epitope of CD36 (GPIIIb), a 85 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. The antibody TR9 cross-blocks binding of FITC-labeled standard antibody OKM5. Anti-CD36 antibodies inhibit adhesive functions (e.g. adherence of infected erythrocytes to target cells).

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Platelets

Preparation

Purified antibody is conjugated with Alexa Fluor^® 700 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

GPIIIb, GPIV, PAS-4, FAT, Thrombospondin receptor, PASIV

Antigen description

CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.

Entrez Gene ID 948

UniProt ID P16671

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD36 (TR9) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD36 positive platelets (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD36 (TR9) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. FASEB J. 2007 Dec 27
PubMed

van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. Biochim Biophys Acta. 2007 Dec 15
PubMed

Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. J Immunol. 2008 Jan 1;180(1):230-7.
PubMed

Product specific references:

Zamora C, Cantó E, Nieto JC, Ortiz MA, Diaz-Torné C, Diaz-Lopez C, Llobet JM, Juarez C, Vidal S: Functional consequences of platelet binding to T lymphocytes in inflammation. J Leukoc Biol. 2013 Sep;94(3):521-9.
PubMed

Bertrand G, Renac V, Lefaix MC, Nivet C, Trudel E, Richard L: Neonatal Intracranial Hemorrhage with a Dramatic Outcome Due to Maternal Anti CD36 Antibodies. Reports 2019, 2(1), 7; doi:10.3390/reports2010007
PubMed

Berre S, Gaudin R, Cunha de Alencar B, Desdouits M, Chabaud M, Naffakh N, Rabaza-Gairi M, Gobert FX, Jouve M, Benaroch P: CD36-specific antibodies block release of HIV-1 from infected primary macrophages and its transmission to T cells. J Exp Med. 2013 Nov 18;210(12):2523-38.
PubMed