Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD355 APC

Anti-Hu CD355 APC

Regulatory status

RUO

Antigen

CD355

Clone

Cr24.1

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-910-T100

In stock

242.00 USD

Variant

100 tests

1A-910-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody Cr24.1 recognizes an extracellular epitope of human CD355, a type I transmembrane glycoprotein, expressed on subsets of CD8+ and CD4+ T cells, on activated NK cells and NKT cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD355-P815 cells

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CRTAM

Antigen description

CD355 is a homodimerizing type I transmembrane glycoprotein, which binds to its ligand SYNCAM (CADM1). CD355 is expressed on subsets of CD8+ and CD4+ T cells, on activated NK cells and NKT cells. Its expression is transient, and reflects its role in cell adhesion and migration.

Entrez Gene ID 56253

UniProt ID O95727

Flow cytometry surface staining pattern of human PMA + Ionomycin stimulated peripheral whole blood stained using anti-human CD355 (Cr24.1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human PMA + Ionomycin stimulated peripheral blood mononuclear cells stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD355 (Cr24.1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 positive CD355 positive cells (red-filled) from CD3 negative CD355 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PMA + Ionomycin stimulated peripheral whole blood stained using anti-human CD355 (Cr24.1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).