Anti-Hu CD355 Alexa Fluor® 488

Anti-Hu CD355 Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
CD355
Clone
Cr24.1
Format
Alexa Fluor® 488
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
A4-910-T100
In stock
248.60 USD
Variant
100 tests
A4-910-T100
In stock
248.60 USD
Product details
Description
Images
SDS download
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody Cr24.1 recognizes an extracellular epitope of human CD355, a type I transmembrane glycoprotein, expressed on subsets of CD8+ and CD4+ T cells, on activated NK cells and NKT cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
CD355-P815 cells
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
CRTAM
Antigen description
CD355 is a homodimerizing type I transmembrane glycoprotein, which binds to its ligand SYNCAM (CADM1). CD355 is expressed on subsets of CD8+ and CD4+ T cells, on activated NK cells and NKT cells. Its expression is transient, and reflects its role in cell adhesion and migration.
Entrez Gene ID 56253
UniProt ID O95727
A4-910_FC_Profil Značení
Flow cytometry surface staining pattern of human stimulated (PMA + Ionomycin) peripheral blood mononuclear cells stained using anti-human CD355 (Cr24.1) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).
A4-910_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human stimulated (PMA + Ionomycin) lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 µl reagent per milion cells in 100 μl of cell suspension) and anti-human CD355 (Cr24.1) Alexa Fluor® 488 antibody (4 µl reagent per milion cells in 100 μl of cell suspension).
A4-910_FC_Histogram
Separation of human CD355 positive CD3 positive T cells (red-filled) from CD355 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + Ionomycin) peripheral blood mononuclear cells stained using anti-human CD355 (Cr24.1) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).
Variant
100 tests
A4-910-T100
In stock
248.60 USD
Variant
100 tests
A4-910-T100
In stock
248.60 USD

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