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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD354 PE
Anti-Hu CD354 PE
Regulatory status
RUO
Antigen
CD354
Clone
6B1
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-933-T100
In stock
248.60 USD
Variant
100 tests
1P-933-T100
In stock
248.60 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 6B1 (also known as 6B1.1G12) recognizes an extracellular epitope of human CD354 (TREM-1), a transmembrane glycoprotein, serving as an important innate immunity receptor.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
recombinant human CD354 fused with IgG
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TREM-1
Antigen description
CD354 (TREM-1), a cell surface glycoprotein expressed mainly on monocytes, macrophages, and neutrophils, but also on e.g. endothelial cells or bronchial epithelium, plays an important role in innate immune responses. Upon triggering by its ligands (mainly bacterial and fungal components, but also some viruses, such as Marburg or Ebola virus), it initiates signaling cascades leading to release of pro-inflammatory cytokines and chemokines. CD354 is strongly present in infectious inflammatory lesions (e.g. in folliculitis or impetigo), but not so much in non-infectious inflammatory lesions, (e.g. vasculitis or psoriasis). However, under certain conditions neutrophils can upregulate CD354 also under non-infectious inflammatory conditions, e.g. in rheumatoid arthritis. Soluble CD354 (sTREM-1) is an important biomarker for identification of septic patients, as well as for prediction of their survival.
Entrez Gene ID
54210
UniProt ID
Q9NP99
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD354 (6B1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD354 (6B1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Product specific references:
Kuemmel A, Alflen A, Schmidt LH, Sebastian M, Wiewrodt R, Schulze AB, Buhl R, Radsak M: Soluble triggering receptor expressed on myeloid cells 1 in lung cancer. Sci Rep. 2018 Jul 17;8(1):10766.
PubMed
Alflen A, Stadler N, Lopez PA, Teschner D, Theobald M, Heß G, Radsak MP: Idelalisib impairs TREM-1 mediated neutrophil inflammatory responses. Sci Rep. 2018 Apr 3;8(1):5558.
PubMed
Prüfer S, Weber M, Sasca D, Teschner D, Wölfel C, Stein P, Stassen M, Schild H, Radsak MP: Distinct signaling cascades of TREM-1, TLR and NLR in neutrophils and monocytic cells. J Innate Immun. 2014;6(3):339-52.
PubMed
Hasibeder A, Stein P, Brandwijk R, Schild H, Radsak MP: Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay. Sci Rep. 2015 Oct 20;5:15381.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
1P-933-T100
In stock
248.60 USD
Variant
100 tests
1P-933-T100
In stock
248.60 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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