Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD354 APC

Anti-Hu CD354 APC

Regulatory status

RUO

Antigen

CD354

Clone

6B1

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-933-T100

In stock

242.00 USD

Variant

100 tests

1A-933-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 6B1 (also known as 6B1.1G12) recognizes an extracellular epitope of human CD354 (TREM-1), a transmembrane glycoprotein, serving as an important innate immunity receptor.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

recombinant human CD354 fused with IgG

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TREM-1

Antigen description

CD354 (TREM-1), a cell surface glycoprotein expressed mainly on monocytes, macrophages, and neutrophils, but also on e.g. endothelial cells or bronchial epithelium, plays an important role in innate immune responses. Upon triggering by its ligands (mainly bacterial and fungal components, but also some viruses, such as Marburg or Ebola virus), it initiates signaling cascades leading to release of pro-inflammatory cytokines and chemokines. CD354 is strongly present in infectious inflammatory lesions (e.g. in folliculitis or impetigo), but not so much in non-infectious inflammatory lesions, (e.g. vasculitis or psoriasis). However, under certain conditions neutrophils can upregulate CD354 also under non-infectious inflammatory conditions, e.g. in rheumatoid arthritis. Soluble CD354 (sTREM-1) is an important biomarker for identification of septic patients, as well as for prediction of their survival.

Entrez Gene ID 54210

UniProt ID Q9NP99

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD354 (6B1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD354 (6B1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Kuemmel A, Alflen A, Schmidt LH, Sebastian M, Wiewrodt R, Schulze AB, Buhl R, Radsak M: Soluble triggering receptor expressed on myeloid cells 1 in lung cancer. Sci Rep. 2018 Jul 17;8(1):10766.
PubMed

Alflen A, Stadler N, Lopez PA, Teschner D, Theobald M, Heß G, Radsak MP: Idelalisib impairs TREM-1 mediated neutrophil inflammatory responses. Sci Rep. 2018 Apr 3;8(1):5558.
PubMed

Prüfer S, Weber M, Sasca D, Teschner D, Wölfel C, Stein P, Stassen M, Schild H, Radsak MP: Distinct signaling cascades of TREM-1, TLR and NLR in neutrophils and monocytic cells. J Innate Immun. 2014;6(3):339-52.
PubMed

Hasibeder A, Stein P, Brandwijk R, Schild H, Radsak MP: Evaluation and validation of the detection of soluble triggering receptor expressed on myeloid cells 1 by enzyme-linked immunosorbent assay. Sci Rep. 2015 Oct 20;5:15381.
PubMed