Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD344 PE

Anti-Hu CD344 PE

Regulatory status

RUO

Antigen

CD344

Clone

CH3A4A7

Format

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-834-T100

In stock

242.00 USD

Variant

100 tests

1P-834-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody CH3A4A7 recognizes an extracellular epitope of CD344 (Frizzled 4), a 7-TM protein of G-protein-coupled receptor family, which is a marker for neuronal stem cells.

Workshop

HLDA Workshop 2006

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

WERI-RB-1 retinoblastoma cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Frizzled-4, FZD4, EVR1, FEVR, GPCR

Antigen description

CD344 (Frizzled class receptor 4) is a G-protein coupled 7-TM protein, predominantly expressed in fetal neuronal progenitor cells, neuronal intestinal cells, as well as in the kidney, lung, brain, and liver. CD344 is important for regulation of cell polarity, proliferation, and tissue development. Defects in CD344 expression, or its mutation, lead e.g. to serious failures in retinal vascularization, defects in cerebellum, progressive hearing loss, or impaired corpora lutea formation and function.

Entrez Gene ID 8322

UniProt ID Q9ULV1

Separation of HeLa cells stained using anti-human CD344 (CH3A4A7) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD344 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.

Product specific references:

Nothelfer K, Obermayr F, Belz N, Reinartz E, Bareiss PM, Bühring HJ, Beschorner R, Just L: Expression of the Wnt Receptor Frizzled-4 in the Human Enteric Nervous System of Infants. Stem Cells Int. 2016;2016:9076823.
PubMed