Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD335 Purified

Anti-Hu CD335 Purified

Regulatory status

RUO

Antigen

CD335

Clone

9E2

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-853-C100

In stock

154.00 USD

Variant

0.1 mg

11-853-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 9E2 recognizes an extracellular epitope of human CD335, a transmembrane glycoprotein, serving as a natural cytotoxicity receptor against virus-infected or cancer cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 µg/ml

Reactivity

Human

Immunogen

fusion protein of human CD335 and Fc part of IgG

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NKP46, NCR1, LY94

Antigen description

CD335 is a type I transmembrane glycoprotein, which serves as one of natural cytotoxicity receptors, and represents major receptor informing NK cells that they should kill particular virus-infected or tumor cell. Its sialic acids residues are involved in recognition of viral hemagglutinins, similarly it recognizes heparan sulphate proteoglycans on the surface of tumor cells. CD335 transfers the signal downstream via association with CD3 zeta chain, that contains intracellular ITAM motifs. Expression of CD335, a specific marker of NK cells (both resting and activated), is down regulated by cortisol, and up-regulated by prolactin.

Entrez Gene ID 9437

UniProt ID O76036

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD335 (9E2) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Separation of lymphocytes stained anti-human CD335 (9E2) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Durrenberger PF, Ettorre A, Kamel F, Webb LV, Sim M, Nicholas RS, Malik O, Reynolds R, Boyton RJ, Altmann DM: Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1). J Neuroinflammation. 2012 Jan 2;9:1. doi: 10.1186/1742-2094-9-1.
PubMed

Alcántara-Hernández M, Leylek R, Wagar LE, Engleman EG, Keler T, Marinkovich MP, Davis MM, Nolan GP, Idoyaga J: High-dimensional phenotypic mapping of human dendritic cells reveals interindividual variation and tissue specialization. Immunity. 2017 Dec 19;47(6):1037-1050.e6.
PubMed

Strittmatter-Keller U, Walter C, Rauld C, Egli N, Regairaz C, Rabe S, Zenke G, Carballido J, Schweighoffer T: Fingerprints of CD8+ T cells on human pre-plasma and memory B cells. PLoS One. 2018 Dec 12;13(12):e0208187.
PubMed

Konnikova L, Boschetti G, Rahman A, Mitsialis V, Lord J, Richmond C, Tomov VT, Gordon W, Jelinsky S, Canavan J, Liss A, Wall S, Field M, Zhou F, Goldsmith JD, Bewtra M, Breault DT, Merad M, Snapper SB: High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue. Mucosal Immunol. 2018 Nov;11(6):1684-1693.
PubMed

Frutoso M, Mair F, Prlic M: OMIP-070: NKp46-Based 27-color phenotyping to define natural killer cells isolated from human tumor tissues. Cytometry A 2020 Sep 26. doi: 10.1002/cyto.a.24230.
PubMed