Anti-Hu CD335 PE

Anti-Hu CD335 PE
Regulatory status
RUO
Antigen
CD335
Clone
9E2
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-853-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 9E2 recognizes an extracellular epitope of human CD335, a transmembrane glycoprotein, serving as a natural cytotoxicity receptor against virus-infected or cancer cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
fusion protein of human CD335 and Fc part of IgG
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
NKP46, NCR1, LY94
Antigen description
CD335 is a type I transmembrane glycoprotein, which serves as one of natural cytotoxicity receptors, and represents major receptor informing NK cells that they should kill particular virus-infected or tumor cell. Its sialic acids residues are involved in recognition of viral hemagglutinins, similarly it recognizes heparan sulphate proteoglycans on the surface of tumor cells. CD335 transfers the signal downstream via association with CD3 zeta chain, that contains intracellular ITAM motifs. Expression of CD335, a specific marker of NK cells (both resting and activated), is down regulated by cortisol, and up-regulated by prolactin.
Entrez Gene ID 9437
UniProt ID O76036
1P-853_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD335 (9E2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-853_FC_Histogram
Separation of CD335 positive lymphocytes (red-filled) from CD335 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD335 (9E2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Durrenberger PF, Ettorre A, Kamel F, Webb LV, Sim M, Nicholas RS, Malik O, Reynolds R, Boyton RJ, Altmann DM: Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1). J Neuroinflammation. 2012 Jan 2;9:1. doi: 10.1186/1742-2094-9-1.
PubMed
Alcántara-Hernández M, Leylek R, Wagar LE, Engleman EG, Keler T, Marinkovich MP, Davis MM, Nolan GP, Idoyaga J: High-dimensional phenotypic mapping of human dendritic cells reveals interindividual variation and tissue specialization. Immunity. 2017 Dec 19;47(6):1037-1050.e6.
PubMed
Strittmatter-Keller U, Walter C, Rauld C, Egli N, Regairaz C, Rabe S, Zenke G, Carballido J, Schweighoffer T: Fingerprints of CD8+ T cells on human pre-plasma and memory B cells. PLoS One. 2018 Dec 12;13(12):e0208187.
PubMed
Konnikova L, Boschetti G, Rahman A, Mitsialis V, Lord J, Richmond C, Tomov VT, Gordon W, Jelinsky S, Canavan J, Liss A, Wall S, Field M, Zhou F, Goldsmith JD, Bewtra M, Breault DT, Merad M, Snapper SB: High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue. Mucosal Immunol. 2018 Nov;11(6):1684-1693.
PubMed
Frutoso M, Mair F, Prlic M: OMIP-070: NKp46-Based 27-color phenotyping to define natural killer cells isolated from human tumor tissues. Cytometry A 2020 Sep 26. doi: 10.1002/cyto.a.24230.
PubMed
Variant
100 tests
1P-853-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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