Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD329 Purified

Anti-Hu CD329 Purified

Regulatory status

RUO

Antigen

CD329

Clone

Format

Purified

Reactivity

Human

Application

ELISA, IP, WB, FC (QC tested), ICC

Variant

0.1 mg

11-885-C100

In stock

154.00 USD

Variant

0.1 mg

11-885-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody K8 recognizes an extracellular epitope of CD239, a type I transmembrane glycoprotein expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells.

Application

ELISA, IP, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

recombinant CD329-Hu IgGFc fusion protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIGLEC9

Antigen description

CD329 is a type I transmembrane glycoprotein of siglec family, which contains intracellular ITIM motif, and a SLAM-like motif, that acts as a docking site for SAP. CD329 can regulate TCR signaling by recruitment of SHP-1, which results in down-regulation of TCR-based gene transcription. It is expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells, weaker expression is detectable in some B cells, NK cells, and T cells.

Entrez Gene ID 27180

UniProt ID Q9Y336

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD329 (K8) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD329 (K8) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Product specific references:

O'Sullivan JA, Carroll DJ, Cao Y, Salicru AN, Bochner BS: Leveraging Siglec-8 endocytic mechanisms to kill human eosinophils and malignant mast cells. J Allergy Clin Immunol. 2018 May;141(5):1774-1785
PubMed

Hudak JE, Canham SM, Bertozzi CR: Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion. Nat Chem Biol. 2014 Jan;10(1):69-75.
PubMed

Avril T, Floyd H, Lopez F, Vivier E, Crocker PR: The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells. J Immunol. 2004 Dec 1;173(11):6841-9.
PubMed