Anti-Hu CD329 Alexa Fluor® 488

Anti-Hu CD329 Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
CD329
Clone
K8
Format
Alexa Fluor® 488
Reactivity
Human
Excitation laser
blue (488 nm)
Variant
100 tests
A4-885-T100
In stock
242.00 USD
Variant
100 tests
A4-885-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody K8 recognizes an extracellular epitope of CD239, a type I transmembrane glycoprotein expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells.
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
recombinant CD329-Hu IgGFc fusion protein
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
SIGLEC9
Antigen description
CD329 is a type I transmembrane glycoprotein of siglec family, which contains intracellular ITIM motif, and a SLAM-like motif, that acts as a docking site for SAP. CD329 can regulate TCR signaling by recruitment of SHP-1, which results in down-regulation of TCR-based gene transcription. It is expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells, weaker expression is detectable in some B cells, NK cells, and T cells.
Entrez Gene ID 27180
UniProt ID Q9Y336
A4-885_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD329 (K8) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A4-885_FC_Histogram
Separation of human monocytes (red-filled) from CD329 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD329 (K8) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

O'Sullivan JA, Carroll DJ, Cao Y, Salicru AN, Bochner BS: Leveraging Siglec-8 endocytic mechanisms to kill human eosinophils and malignant mast cells. J Allergy Clin Immunol. 2018 May;141(5):1774-1785
PubMed
Hudak JE, Canham SM, Bertozzi CR: Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion. Nat Chem Biol. 2014 Jan;10(1):69-75.
PubMed
Avril T, Floyd H, Lopez F, Vivier E, Crocker PR: The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells. J Immunol. 2004 Dec 1;173(11):6841-9.
PubMed
Variant
100 tests
A4-885-T100
In stock
242.00 USD
Variant
100 tests
A4-885-T100
In stock
242.00 USD

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