Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD328 Purified

Anti-Hu CD328 Purified

Regulatory status

RUO

Antigen

CD328

Clone

6-434

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-799-C100

Ask for delivery term HERE

143.00 USD

0.025 mg

11-799-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-799-C100

Ask for delivery term HERE

143.00 USD

0.025 mg

11-799-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 6-434 recognizes an extracellular epitope of CD328 (Siglec-7), a 75 kDa transmembrane glycoprotein expressed mainly on NK cells, dendritic cells and monocytes.

Workshop

HLDA VIII: WS Code ARN-80652

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

human dendritic cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIGLEC7, p75/AIRM1, D-siglec

Antigen description

CD328, also known as Siglec-7 or p75/AIRM1, is a 75 kDa type I transmembrane glycoprotein of sialic acid-binding immunoglobulin-like lectin (Siglec) family. CD328 binds to sialylated glycans with alpha2,6 sialyl and alpha2,8 disyalyl residues and mediates sialic acid-dependent cell-cell binding. As it contains in its intracellular part the immunoreceptor tyrosine-based inhibitory motif (ITIM), it serves as an inhibitory receptor, e.g. of NK cells.

Entrez Gene ID 27036

UniProt ID Q9Y286

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD328 (6-434) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Separation of human CD328 positive lymphocytes (red-filled) from CD328 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD328 (6-434) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Product specific references:

Bradley T, Peppa D, Pedroza-Pacheco I, Li D, Cain DW, Henao R, Venkat V, Hora B, Chen Y, Vandergrift NA, Overman RG, Edwards RW, Woods CW, Tomaras GD, Ferrari G, Ginsburg GS, Connors M, Cohen MS, Moody MA, Borrow P, Haynes BF: RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018 Oct 4;175(2):387-399
PubMed