Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD328 PE

Anti-Hu CD328 PE

Regulatory status

RUO

Antigen

CD328

Clone

6-434

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-799-T100

In stock

220.00 USD

25 tests

1P-799-T025

Delivery 1 week

110.00 USD

Variant

100 tests

1P-799-T100

In stock

220.00 USD

25 tests

1P-799-T025

Delivery 1 week

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 6-434 recognizes an extracellular epitope of CD328 (Siglec-7), a 75 kDa transmembrane glycoprotein expressed mainly on NK cells, dendritic cells and monocytes.

Workshop

HLDA VIII: WS Code ARN-80652

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human dendritic cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SIGLEC7, p75/AIRM1, D-siglec

Antigen description

CD328, also known as Siglec-7 or p75/AIRM1, is a 75 kDa type I transmembrane glycoprotein of sialic acid-binding immunoglobulin-like lectin (Siglec) family. CD328 binds to sialylated glycans with alpha2,6 sialyl and alpha2,8 disyalyl residues and mediates sialic acid-dependent cell-cell binding. As it contains in its intracellular part the immunoreceptor tyrosine-based inhibitory motif (ITIM), it serves as an inhibitory receptor, e.g. of NK cells.

Entrez Gene ID 27036

UniProt ID Q9Y286

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD328 (6-434) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human CD328 (6-434) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD328 positive CD56 positive NK cells (red-filled) from CD328 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD328 (6-434) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Bradley T, Peppa D, Pedroza-Pacheco I, Li D, Cain DW, Henao R, Venkat V, Hora B, Chen Y, Vandergrift NA, Overman RG, Edwards RW, Woods CW, Tomaras GD, Ferrari G, Ginsburg GS, Connors M, Cohen MS, Moody MA, Borrow P, Haynes BF: RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018 Oct 4;175(2):387-399
PubMed