Anti-Hu CD326 Purified

Anti-Hu CD326 Purified
Regulatory status
RUO
Antigen
CD326
Clone
VU-1D9
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-581-C100
In stock
143.00 USD

0.025 mg
11-581-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-581-C100
In stock
143.00 USD

0.025 mg
11-581-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin.
Immunocytochemistry: Recommended dilution: 4 μg/ml.
Western blotting: Recommended dilution: 1-4 μg/ml, non-reducing conditions.
Flow cytometry: Recommended dilution: 1-3 μg/ml.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
Reactivity
Human
Immunogen
Small cell lung carcinoma cell line H69.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1
Antigen description
CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Entrez Gene ID 4072
UniProt ID P16422
11-581_WB
Western blotting analysis of human CD326 using mouse monoclonal antibody VU-1D9 on lysates of MCF-7 cells and HEK293T cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD326 was detected around 40 kDa.
11-581_FC_Histogram
Separation of MCF-7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) of cell lines stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 μg/ml) GAM APC.
11-581_FC_Profil Značení
Flow cytometry surface staining pattern of MCF-7 cells stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 μg/ml) GAM APC.
11-581_IHC
Immunohistochemistry staining of human prostate (paraffin sections) using anti-CD326 (VU-1D9). Commercially tested by LifeSpan BioSciences.

Product specific references:

Brunner A, Prelog M, Verdorfer I, Tzankov A, Mikuz G, Ensinger C: EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 2008 Mar;61(3):307-10.
PubMed
Winter MJ, Nagtegaal ID, van Krieken JH, Litvínov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163(6):2139-48.
PubMed
Li G, Passebosc-Faure K, Lambert C, Gentil-Perret A, Blanc F, Oosterwijk E, Mosnier JF, Genin C, Tostain J: Flow cytometric analysis of antigen expression in malignant and normal renal cells. Anticancer Res. 2000 Jul-Aug;20(4):2773-8.
PubMed
Ogura E, Senzaki H, Yoshizawa K, Hioki K, Tsubura A: Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors. Anticancer Res. 1998 Sep-Oct;18(5B):3669-75.
PubMed
Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S: Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors. J Cutan Pathol. 1992 Feb;19(1):73-9.
PubMed
Variant
0.1 mg
11-581-C100
In stock
143.00 USD

0.025 mg
11-581-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-581-C100
In stock
143.00 USD

0.025 mg
11-581-C025
Delivery 1 week
71.50 USD

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