Anti-Hu CD326 Purified

Anti-Hu CD326 Purified
Regulatory status
RUO
Antigen
CD326
Clone
323/A3
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-582-C100
In stock
130.00 USD

0.025 mg
11-582-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-582-C100
In stock
130.00 USD

0.025 mg
11-582-C025
Delivery 1 week
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 323/A3 recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.
Application details
Western blotting: Non-reducing conditions; recommended dilution: 1-2 μg/ml; positive control: MCF-7 cells.
Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma.
Immunocytochemistry: Recommended dilution: 1-10 μg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Reactivity
Human
Negative species
Rat
Immunogen
Human breast cancer MCF-7 cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1
Antigen description
CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Entrez Gene ID 4072
UniProt ID P16422
11-582_WB_3
Western blotting analysis of human CD326 (EpCAM) using mouse monoclonal antibody 323/A3 on lysates of MCF-7 cell line and HEK293T/17 cell line (CD326 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD326 monoclonal antibody 323/A3 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD326 protein at approximately 40 kDa.
11-582_FC_Histogram
Separation of MCF-7 cells (red-filled) from Jurkat cells (black-dashed) in flow cytometry analysis (surface staining) of cellular suspensions of Jurkat and MCF-7 cell lines stained using anti-human CD326 (323/A3) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Product specific references:

Kirman I, Jenkins D, Fowler R, Whelan RL: Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM). Dig Dis Sci. 2003 Dec;48(12):2306-9.
PubMed
Winter MJ, Nagtegaal ID, van Krieken JH, Litvínov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163(6):2139-48.
PubMed
Balzar M, Briaire-de Bruijn IH, Rees-Bakker HA, Prins FA, Helfrich W, de Leij L, Riethmüller G, Alberti S, Warnaar SO, Fleuren GJ, Litvínov SV: Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol Cell Biol. 2001 Apr;21(7):2570-80.
PubMed
Balzar M, Bakker HA, Briaire-de-Bruijn IH, Fleuren GJ, Warnaar SO, Litvinov SV: Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule. Mol Cell Biol. 1998 Aug;18(8):4833-43.
PubMed
Litvínov SV, Bakker HA, Gourevitch MM, Velders MP, Warnaar SO: Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 1994 Oct;2(5):417-28.
PubMed
Litvínov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO: Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 1994 Apr;125(2):437-46.
PubMed
Edwards DP, Grzyb KT, Dressler LG, Mansel RE, Zava DT, Sledge GW Jr, McGuire WL: Monoclonal antibody identification and characterization of a Mr 43,000 membrane glycoprotein associated with human breast cancer. Cancer Res. 1986 Mar;46(3):1306-17.
PubMed
Variant
0.1 mg
11-582-C100
In stock
130.00 USD

0.025 mg
11-582-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-582-C100
In stock
130.00 USD

0.025 mg
11-582-C025
Delivery 1 week
65.00 USD

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