Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD32 Purified

Anti-Hu CD32 Purified

Regulatory status

RUO

Antigen

CD32

Clone

3D3

Format

Purified

Reactivity

Human

Application

MC (CyTOF), FC (QC tested)

Variant

0.1 mg

11-751-C100

In stock

143.00 USD

0.025 mg

11-751-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-751-C100

In stock

143.00 USD

0.025 mg

11-751-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

Application

MC (CyTOF), FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-6 μg/ml.

Reactivity

Human

Immunogen

purified glycosylated recombinant human FcgammaRIIa2

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FCG2, FCGR2A, IGFR2

Antigen description

CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).

Entrez Gene ID 2212

UniProt ID P12318

Mass cytometry (surface staining) of human peripheral blood with anti-CD32 (3D3) 167Er. Gated on singlets.

Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD32 (3D3) purified antibody (concentration in sample 1.78 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD32 (3D3) purified antibody (concentration in sample 1.78 μg/ml, GAM APC).

Product specific references:

Dutertre CA, Bonnin-Gélizé E, Pulford K, Bourel D, Fridman WH, Teillaud JL: A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function. J Leukoc Biol. 2008 Dec;84(6):1511-20
PubMed

Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 15;4(3):566-77.
PubMed

Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA: Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood. 2009 Jul 9;114(2):318-27.
PubMed

Vely F, Gruel N, Moncuit J, Cochet O, Rouard H, Dare S, Galon J, Sautes C, Fridman WH, Teillaud JL: A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays. Hybridoma. 1997 Dec;16(6):519-28.
PubMed

Boruchov AM, Heller G, Veri MC, Bonvini E, Ravetch JV, Young JW: Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions. J Clin Invest. 2005 Oct;115(10):2914-23.
PubMed