Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD319 FITC

Anti-Hu CD319 FITC

Regulatory status

RUO

Antigen

CD319

Clone

162

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-908-T100

In stock

198.00 USD

Variant

100 tests

1F-908-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody 162 recognizes an extracellular epitope of human CD319, a transmembrane glycoprotein expressed by cytotoxic T cells, activated B cells, NK cells, and mature dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Recombinant extracellular domain of human CD319 (204 aa) fused with Fc part of human IgG1

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SLAMF7, 19A, CS1, CRACC

Antigen description

CD319 is a homophilic type I transmembrane protein with two intracellular immunoreceptor tyrosine-based switch motives (ITSMs). It is expressed by cytotoxic T cells, activated B cells and plasma cells, NK cells, and mature dendritic cells. CD319 triggers NK cell-mediated cytotoxicity. Ligation of CD319 can induce proliferation, cell adhesion, cytokine secretion, and cytotoxicity of T cells and NK cells.

Entrez Gene ID 57823

UniProt ID Q9NQ25

Anti-human CD319 FITC antibody (clone 162) works in Flow Cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD319 FITC antibody (clone 162) was used in concentration 2 µg/ml in stained blood sample (2 x 10⁶ cells).

Staining pattern of Anti-human CD319 FITC antibody (clone 162) in Dot-plot fluorescence visualization (B cell gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD319 FITC antibody (clone 162) was used in concentration 2 µg/ml in stained stained blood sample (2 x 10⁶ cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) in concentration 2 µg/ml, respectively.

Staining pattern of Anti-human CD319 FITC antibody (clone 162) in Dot-plot fluorescence visualization (B cell gate)

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Mouse monoclonal anti-human CD319 FITC antibody (clone 162) was used in concentration 2 µg/ml in stained stained blood sample (2 x 10⁶ cells) and Mouse monoclonal anti-human CD138 Pacific Blue™ antibody (clone MI15) in concentration 2 µg/ml, respectively.

Reactivity of Anti-human CD319 FITC antibody (clone 162) on human peripheral leukocytes

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats.
Suspension of blood leukocytes (2 x 10⁶ cells) was added to the mixture of CD319 FITC antibody (clone 162, 2 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Product specific references:

Chan WK, Kang S, Youssef Y, Glankler EN, Barrett ER, Carter AM, Ahmed EH, Prasad A, Chen L, Zhang J, Benson DM Jr, Caligiuri MA, Yu J: A CS1-NKG2D bispecific antibody collectively activates cytolytic immune cells against multiple myeloma. Cancer Immunol Res. 2018 Jul;6(7):776-787.
PubMed