Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD319 Alexa Fluor<sup>®</sup> 647

Anti-Hu CD319 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD319

Clone

162

Format

Alexa Fluor^® 647

Reactivity

Human

Application

FC (QC tested), IHC-P

Excitation laser

red (633 nm)

Variant

100 tests

A6-908-T100

In stock

242.00 USD

Variant

100 tests

A6-908-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody 162 recognizes an extracellular epitope of human CD319, a transmembrane glycoprotein expressed by cytotoxic T cells, activated B cells, NK cells, and mature dendritic cells.

Application

FC (QC tested), IHC-P

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Recombinant extracellular domain of human CD319 (204 aa) fused with Fc part of human IgG1

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

SLAMF7, 19A, CS1, CRACC

Antigen description

CD319 is a homophilic type I transmembrane protein with two intracellular immunoreceptor tyrosine-based switch motives (ITSMs). It is expressed by cytotoxic T cells, activated B cells and plasma cells, NK cells, and mature dendritic cells. CD319 triggers NK cell-mediated cytotoxicity. Ligation of CD319 can induce proliferation, cell adhesion, cytokine secretion, and cytotoxicity of T cells and NK cells.

Entrez Gene ID 57823

UniProt ID Q9NQ25

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD319 (162) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD319 (162) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD319 positive CD56 positive NK cells (red-filled) from CD319 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD319 (162) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Chan WK, Kang S, Youssef Y, Glankler EN, Barrett ER, Carter AM, Ahmed EH, Prasad A, Chen L, Zhang J, Benson DM Jr, Caligiuri MA, Yu J: A CS1-NKG2D bispecific antibody collectively activates cytolytic immune cells against multiple myeloma. Cancer Immunol Res. 2018 Jul;6(7):776-787.
PubMed