Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD318 PE

Anti-Hu CD318 PE

Regulatory status

RUO

Antigen

CD318

Clone

CUB1

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-833-C100

In stock

242.00 USD

Variant

0.1 mg

1P-833-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody CUB1 recognizes an extracellular epitope of CD318, a type I transmembrane protein involved in early hematopoiesis.

Workshop

HCDM listed

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

NIH-3T3/CD318 cells

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CDCP1, SIMA135, TRASK

Antigen description

CD318 (CUB domain containing protein 1) is a complement domains-containing transmembrane glycoprotein, which takes part in early hematopoiesis. It is expressed on CD34+CD133+ bone marrow cells, keratinocytes, and in human colorectal and breast cancers. It is being used as a marker of mesenchymal stem-like cells, neural progenitor cells, and also as an independent marker for the diagnosis of myeloid leukemias.

Entrez Gene ID 64866

UniProt ID Q9H5V8

Separation of HT-29 cells stained using anti-human CD318 (CUB1) PE antibody (concentration in sample 5 μg/ml, red-filled) from HT-29 cells stained using mouse IgG2b isotype control (MPC-11) PE antibody (concentration in sample 5 μg/ml, same as CD318 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HT-29 cell suspension.

Product specific references:

Battula VL, Treml S, Bareiss PM, Gieseke F, Roelofs H, de Zwart P, Müller I, Schewe B, Skutella T, Fibbe WE, Kanz L, Bühring HJ: Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem cell antigen-1. Haematologica. 2009 Feb;94(2):173-84.
PubMed

Bühring HJ, Kuçi S, Conze T, Rathke G, Bartolović K, Grünebach F, Scherl-Mostageer M, Brümmendorf TH, Schweifer N, Lammers R: CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and nonhematopoietic origin. Stem Cells. 2004;22(3):334-43.
PubMed