Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD316 PE

Anti-Hu CD316 PE

Regulatory status

RUO

Antigen

CD316

Clone

8A12

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-940-T100

In stock

220.00 USD

Variant

100 tests

1P-940-T100

In stock

220.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody 8A12 recognizes an extracellular epitope of CD316, a type I transmembrane glycoprotein, expressed mainly by lymphocytes, NK cells and in a broad range of tissues.

Workshop

HCDM listed

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human leukocytes

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

EWI2, PGRL, KCT-4, LIR-D1, IGSF8

Antigen description

CD316 is an approximately 63 kDa transmembrane glycoprotein, which forms stable complexes with CD9, as well as with CD81. It seems that CD316 participates in regulation of cell polarity and motility, in cell proliferation, oocyte fertilization and tumor cell metastasis. It is expressed mainly by lymphocytes, NK cells and in a broad range of tissues, and associates with actin cytoskeleton.

Entrez Gene ID 93185

UniProt ID Q969P0

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD316 (8A12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD316 (8A12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Gordón-Alonso M, Sala-Valdés M, Rocha-Perugini V, Pérez-Hernández D, López-Martín S, Ursa A, Alvarez S, Kolesnikova TV, Vázquez J, Sánchez-Madrid F, Yáñez-Mó M: EWI-2 association with α-actinin regulates T cell immune synapses and HIV viral infection. J Immunol. 2012 Jul 15;189(2):689-700.
PubMed