Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD31 Pacific Blue™

Anti-Hu CD31 Pacific Blue™

Regulatory status

RUO

Antigen

CD31

Clone

MEM-05

Format

Pacific Blue™

Reactivity

Human

Application

FC (QC tested)

Excitation laser

violet (405 nm)

Variant

100 tests

PB-273-T100

In stock

220.00 USD

25 tests

PB-273-T025

Delivery 1 week

110.00 USD

Variant

0.1 mg

11-261-C100

In stock

220.00 USD

0.1 mg

11-261-C100

In stock

110.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Leukocytes of a patient suffering from LGL-type leukaemia

Preparation

Purified antibody is conjugated with Pacific Blue™ NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

PECAM-1, EndoCAM, GPIIA, PECA1

Antigen description

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Entrez Gene ID 5175

UniProt ID P16284

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD31 (MEM-05) Pacific Blue™.

General references:

Wong MX, Hayball JD, Jackson DE: PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol. 2007 Oct 29
PubMed

Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2514-23.
PubMed

Wee JL, Jackson DE: The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1;106(12):3816-23.
PubMed

Jackson DE: The unfolding tale of PECAM-1. FEBS Lett. 2003 Apr 10;540(1-3):7-14.
PubMed

Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) acts as a regulator of B-cell development, B-cell antigen receptor (BCR)-mediated activation, and autoimmune disease. Blood. 2002 Jul 1;100(1):184-93.
PubMed

Newman DK, Hamilton C, Newman PJ: Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck). Blood. 2001 Apr 15;97(8):2351-7.
PubMed

Product specific references:

Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15;166(4):2364-71.
PubMed

Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS One. 2012;7(12):e52815. doi: 10.1371/journal.pone.0052815.
PubMed

Kalina T, Bakardjieva M, Blom M, Perez-Andres M, Barendregt B, Kanderová V, Bonroy C, Philippé J, Blanco E, Pico-Knijnenburg I, Paping JH, Wolska-Kuśnierz B, Pac M, Tkazcyk J, Haerynck F, Akar HH, Formánková R, Freiberger T, Svatoň M, Šedivá A, Arriba-Méndez S, Orfao A, van Dongen JJ, van der Burg M: EuroFlow standardized approach to diagnostic immunopheneotyping of severe PID innNewborns and young children. Front Immunol. 2020 Mar 19;11:371.
PubMed

Wei F, Cao S, Ren X, Liu H, Yu J, Li H, Hao X: Efficient antiproliferative and antiangiogenic effects on human ovarian cancer growth by gene transfer of attenuated mutants of Shiga-like toxin I. Int J Gynecol Cancer. Jul-Aug 2008;18(4):677-91.
PubMed

Variant

100 tests

PB-273-T100

In stock

220.00 USD

25 tests

PB-273-T025

Delivery 1 week

110.00 USD

Variant

0.1 mg

11-261-C100