Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD307c PE

Anti-Hu CD307c PE

Regulatory status

RUO

Antigen

CD307c

Clone

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-858-T100

In stock

242.00 USD

Variant

100 tests

1P-858-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody H5 recognizes an epitope within extracellular part of CD307c, a transmembrane glycoprotein expressed mainly on NK cells, and T and B cell subsets.

Workshop

HLDA IX

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

DNA-immunization followed by a boost with CD307c transfected cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FcRL3, IRTA3

Antigen description

CD307c is a type I transmembrane glycoprotein of the Fc receptor family. It contains both ITAM and ITIM motifs in its cytoplasmic domain. CD307c is expressed on the surface of NK cells, and T, Treg, B and plasma cell subsets. It seems to play a role in the regulation of immune response. Defects in CD307c function can result in autoimmune diseases, e.g. rheumatoid arthritis or systemic lupus erythematosus.

Entrez Gene ID 115352

UniProt ID Q96P31

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307c (H5) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307c (H5) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD307c positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307c (H5) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Jourdan M, Robert N, Cren M, Thibaut C, Duperray C, Kassambara A, Cogné M, Tarte K, Klein B, Moreaux J: Characterization of human FCRL4-positive B cells. PLoS One. 2017 Jun 21;12(6):e0179793.
PubMed

Wysocka M, Kossenkov AV, Benoit BM, Troxel AB, Singer E, Schaffer A, Kim B, Dentchev T, Nagata S, Ise T, Showe LC, Rook AH: CD164 and FCRL3 are highly expressed on CD4+CD26- T cells in Sézary syndrome patients. J Invest Dermatol. 2014 Jan;134(1):229-236.
PubMed

Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26
PubMed