Anti-Hu CD307a Purified

Anti-Hu CD307a Purified
Regulatory status
RUO
Antigen
CD307a
Clone
E3
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-860-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody E3 recognizes an extracellular epitope of CD307a, a transmembrane glycoprotein expressed predominantly on the surface of mature B cells.
Workshop
HLDA IX
Application
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
DNA-immunization followed by a boost with the CD307a transfected cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
FcRL1, FcRH1, IRTA5
Antigen description
CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls.
Entrez Gene ID 115350
UniProt ID Q96LA6
11-860_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD307a (E3) purified antibody (5 μg/ml, GAM-FITC).

Product specific references:

Li H, Borrego F, Nagata S, Tolnay M: Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016 May 15;196(10):4064-74.
PubMed
Du X, Nagata S, Ise T, Stetler-Stevenson M, Pastan I: FCRL1 on chronic lymphocytic leukemia, hairy cell leukemia, and B-cell non-Hodgkin lymphoma as a target of immunotoxins. Blood. 2008 Jan 1;111(1):338-43
PubMed
Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26
PubMed
Variant
0.1 mg
11-860-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD