Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD307a PE

Anti-Hu CD307a PE

Regulatory status

RUO

Antigen

CD307a

Clone

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-860-T100

Ask for delivery term HERE

248.60 USD

Variant

100 tests

1P-860-T100

Ask for delivery term HERE

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody E3 recognizes an extracellular epitope of CD307a, a transmembrane glycoprotein expressed predominantly on the surface of mature B cells.

Workshop

HLDA IX

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

DNA-immunization followed by a boost with the CD307a transfected cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FcRL1, FcRH1, IRTA5

Antigen description

CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls.

Entrez Gene ID 115350

UniProt ID Q96LA6

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307a(E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (4G7) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Li H, Borrego F, Nagata S, Tolnay M: Fc receptor-like 5 expression distinguishes two distinct subsets of human circulating tissue-like memory B cells. J Immunol. 2016 May 15;196(10):4064-74.
PubMed

Du X, Nagata S, Ise T, Stetler-Stevenson M, Pastan I: FCRL1 on chronic lymphocytic leukemia, hairy cell leukemia, and B-cell non-Hodgkin lymphoma as a target of immunotoxins. Blood. 2008 Jan 1;111(1):338-43
PubMed

Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26
PubMed