Anti-Hu CD307a PE

Anti-Hu CD307a PE
Regulatory status
RUO
Antigen
CD307a
Clone
E3
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-860-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody E3 recognizes an extracellular epitope of CD307a, a transmembrane glycoprotein expressed predominantly on the surface of mature B cells.
Workshop
HLDA IX
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
DNA-immunization followed by a boost with the CD307a transfected cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
FcRL1, FcRH1, IRTA5
Antigen description
CD307a is a type I transmembrane glycoprotein of the Fc receptor family. It contains two ITAM motifs in its cytoplasmic domain. CD307a is expressed mainly on the surface of mature B-cells, and is down-regulated in germinal center B-cells. Expression of CD307a is higher in patients with autoimmune diseases, compared with healthy controls.
Entrez Gene ID 115350
UniProt ID Q96LA6
1P-860_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307a (E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-860_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307a(E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (4G7) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Li H, Borrego F, Nagata S, Tolnay M: Fc receptor-like 5 expression distinguishes two distinct subsets of human circulating tissue-like memory B cells. J Immunol. 2016 May 15;196(10):4064-74.
PubMed
Du X, Nagata S, Ise T, Stetler-Stevenson M, Pastan I: FCRL1 on chronic lymphocytic leukemia, hairy cell leukemia, and B-cell non-Hodgkin lymphoma as a target of immunotoxins. Blood. 2008 Jan 1;111(1):338-43
PubMed
Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26
PubMed
Variant
100 tests
1P-860-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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