Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD305 Purified

Anti-Hu CD305 Purified

Regulatory status

RUO

Antigen

CD305

Clone

NKTA255

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-713-C100

In stock

143.00 USD

0.025 mg

11-713-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-713-C100

In stock

143.00 USD

0.025 mg

11-713-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.

Workshop

HCDM listed

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Activated NK cells and CD3- thymocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LAIR1

Antigen description

CD305, also known as LAIR1 (leukocyte-associated Ig-like receptor 1), is an inhibitory receptor found on many types of peripheral blood cells. It serves to suppress cell cytotoxicity, activation, proliferation, and differentiation regarding autoantigens via its two intracellular ITIM sites. CD305 belongs to the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family of proteins. It reacts with collagen ligands.

Entrez Gene ID 3903

UniProt ID Q6GTX8

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 μg/ml, GAM APC) and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD305 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD305 (NKTA255) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Product specific references:

Colombo BM, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi MR, Poggi A: Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS One. 2012;7(2):e31903
PubMed

Poggi A, Tomasello E, Ferrero E, Zocchi MR, Moretta L: p40/LAIR-1 regulates the differentiation of peripheral blood precursors to dendritic cells induced by granulocyte-monocyte colony-stimulating factor. Eur J Immunol. 1998 Jul;28(7):2086-91.
PubMed

Poggi A, Tomasello E, Revello V, Nanni L, Costa P, Moretta L: p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes. Int Immunol. 1997 Sep;9(9):1271-9.
PubMed

Meyaard L: The inhibitory collagen receptor LAIR-1 (CD305). J Leukoc Biol. 2008 Apr;83(4):799-803.
PubMed

Son M, Santiago-Schwarz F, Al-Abed Y, Diamond B: C1q limits dendritic cell differentiation and activation by engaging LAIR-1. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3160-7
PubMed

Poggi A, Pella N, Morelli L, Spada F, Revello V, Sivori S, Augugliaro R, Moretta L, Moretta A: p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol. 1995 Feb;25(2):369-76.
PubMed

Warren HS: The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report. Cell Immunol. 2005 Jul-Aug;236(1-2):17-20.
PubMed