Anti-Hu CD305 PerCP-Cy™5.5

Anti-Hu CD305 PerCP-Cy™5.5
Regulatory status
RUO
Antigen
CD305
Clone
NKTA255
Format
PerCP-Cy™5.5
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T9-713-T100
In stock
330.00 USD

25 tests
T9-713-T025
Delivery 1 week
165.00 USD
Variant
100 tests
T9-713-T100
In stock
330.00 USD

25 tests
T9-713-T025
Delivery 1 week
165.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.
Workshop
HCDM listed
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Activated NK cells and CD3- thymocytes
Preparation
Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
LAIR1
Antigen description
CD305, also known as LAIR1 (leukocyte-associated Ig-like receptor 1), is an inhibitory receptor found on many types of peripheral blood cells. It serves to suppress cell cytotoxicity, activation, proliferation, and differentiation regarding autoantigens via its two intracellular ITIM sites. CD305 belongs to the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family of proteins. It reacts with collagen ligands.
Entrez Gene ID 3903
UniProt ID Q6GTX8
T9-713 FC
Flow cytometry analysis (surface staining) of CD305 / LAIR-1 in human peripheral blood (lymphocyte gate) with anti-CD305 (NKTA255) PerCP-Cy™5.5.

Product specific references:

Colombo BM, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi MR, Poggi A: Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS One. 2012;7(2):e31903
PubMed
Poggi A, Tomasello E, Ferrero E, Zocchi MR, Moretta L: p40/LAIR-1 regulates the differentiation of peripheral blood precursors to dendritic cells induced by granulocyte-monocyte colony-stimulating factor. Eur J Immunol. 1998 Jul;28(7):2086-91.
PubMed
Poggi A, Tomasello E, Revello V, Nanni L, Costa P, Moretta L: p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes. Int Immunol. 1997 Sep;9(9):1271-9.
PubMed
Meyaard L: The inhibitory collagen receptor LAIR-1 (CD305). J Leukoc Biol. 2008 Apr;83(4):799-803.
PubMed
Son M, Santiago-Schwarz F, Al-Abed Y, Diamond B: C1q limits dendritic cell differentiation and activation by engaging LAIR-1. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3160-7
PubMed
Poggi A, Pella N, Morelli L, Spada F, Revello V, Sivori S, Augugliaro R, Moretta L, Moretta A: p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol. 1995 Feb;25(2):369-76.
PubMed
Warren HS: The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report. Cell Immunol. 2005 Jul-Aug;236(1-2):17-20.
PubMed
Variant
100 tests
T9-713-T100
In stock
330.00 USD

25 tests
T9-713-T025
Delivery 1 week
165.00 USD
Variant
100 tests
T9-713-T100
In stock
330.00 USD

25 tests
T9-713-T025
Delivery 1 week
165.00 USD

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