Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD305 Alexa Fluor® 488

Anti-Hu CD305 Alexa Fluor® 488

Anti-Hu CD305 Alexa Fluor® 488
Regulatory status
RUO
Antigen
CD305
Clone
NKTA255
Format
Alexa Fluor® 488
Reactivity
Human
Application
WB, FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
A4-713-T100
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.
Application
WB, FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Activated NK cells and CD3- thymocytes
Other names
LAIR1
Preparation
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A4-713_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A4-713_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
A4-713_FC_Histogram
Separation of human CD305 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Colombo BM, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi MR, Poggi A: Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS One. 2012;7(2):e31903
PubMed
Poggi A, Tomasello E, Ferrero E, Zocchi MR, Moretta L: p40/LAIR-1 regulates the differentiation of peripheral blood precursors to dendritic cells induced by granulocyte-monocyte colony-stimulating factor. Eur J Immunol. 1998 Jul;28(7):2086-91.
PubMed
Poggi A, Tomasello E, Revello V, Nanni L, Costa P, Moretta L: p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes. Int Immunol. 1997 Sep;9(9):1271-9.
PubMed
Meyaard L: The inhibitory collagen receptor LAIR-1 (CD305). J Leukoc Biol. 2008 Apr;83(4):799-803.
PubMed
Son M, Santiago-Schwarz F, Al-Abed Y, Diamond B: C1q limits dendritic cell differentiation and activation by engaging LAIR-1. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3160-7
PubMed
Poggi A, Pella N, Morelli L, Spada F, Revello V, Sivori S, Augugliaro R, Moretta L, Moretta A: p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol. 1995 Feb;25(2):369-76.
PubMed
Warren HS: The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report. Cell Immunol. 2005 Jul-Aug;236(1-2):17-20.
PubMed
Variant
100 tests
A4-713-T100
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Contact distributor
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