Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD305 Alexa Fluor® 488

Anti-Hu CD305 Alexa Fluor® 488

Regulatory status

RUO

Antigen

CD305

Clone

NKTA255

Format

Alexa Fluor® 488

Reactivity

Human

Application

WB, FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

A4-713-T100

Out of stock

220.00 USD

Variant

0.1 mg

11-261-C100

In stock

220.00 USD

Contact distributor

Product details

Images

References

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody NKTA255 recognizes an extracellular epitope of CD305 / LAIR1, a 40 kDa type I transmembrane glycoprotein expressed on NK, T, and B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells, CD34+ hematopoietic progenitor cells and thymocytes.

Workshop

HLDA X

Application

WB, FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Activated NK cells and CD3- thymocytes

Other names

LAIR1

Preparation

The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

Storage and handling

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD305 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD305 (NKTA255) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Colombo BM, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi MR, Poggi A: Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS One. 2012;7(2):e31903
PubMed

Poggi A, Tomasello E, Ferrero E, Zocchi MR, Moretta L: p40/LAIR-1 regulates the differentiation of peripheral blood precursors to dendritic cells induced by granulocyte-monocyte colony-stimulating factor. Eur J Immunol. 1998 Jul;28(7):2086-91.
PubMed

Poggi A, Tomasello E, Revello V, Nanni L, Costa P, Moretta L: p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes. Int Immunol. 1997 Sep;9(9):1271-9.
PubMed

Meyaard L: The inhibitory collagen receptor LAIR-1 (CD305). J Leukoc Biol. 2008 Apr;83(4):799-803.
PubMed

Son M, Santiago-Schwarz F, Al-Abed Y, Diamond B: C1q limits dendritic cell differentiation and activation by engaging LAIR-1. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):E3160-7
PubMed

Poggi A, Pella N, Morelli L, Spada F, Revello V, Sivori S, Augugliaro R, Moretta L, Moretta A: p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol. 1995 Feb;25(2):369-76.
PubMed

Warren HS: The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report. Cell Immunol. 2005 Jul-Aug;236(1-2):17-20.
PubMed

Variant

100 tests

A4-713-T100

Out of stock

220.00 USD

Variant

0.1 mg

11-261-C100

In stock

220.00 USD

Contact distributor

Datasheet download