Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD300e Purified Low Endotoxin

Anti-Hu CD300e Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD300e

Clone

UP-H2

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, WB, FC (QC tested), FUNC

Variant

0.1 mg

12-728-C100

In stock

147.40 USD

Variant

0.1 mg

12-728-C100

In stock

147.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.

Application

IP, WB, FC (QC tested), FUNC

Application details

Functional application: Stimulation.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

CD300e-HA-transfected cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2

Antigen description

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Entrez Gene ID 342510

UniProt ID Q496F6

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD300e (UP-H2) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from CD300e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD300e (UP-H2) purified antibody (concentration in sample 4 μg/ml, GAM APC).

General references:

Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30(5):209-17.
PubMed

Product specific references:

Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M: Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004 Dec 1;173(11):6703-11.
PubMed

Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M: Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells. Eur J Immunol. 2010 Mar;40(3):722-32.
PubMed