Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD300e Purified Low Endotoxin

Anti-Hu CD300e Purified Low Endotoxin

Anti-Hu CD300e Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD300e
Clone
UP-H2
Format
Purified Low Endotoxin
Reactivity
Human
Application
IP, WB, FC (QC tested), FUNC
Variant
0.1 mg
12-728-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Contact distributor
Product details
Description
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
Application
IP, WB, FC (QC tested), FUNC
Application details
Functional application: Stimulation.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
CD300e-HA-transfected cells
Other names
CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2
Antigen description
CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.
Entrez Gene ID 342510
UniProt ID Q496F6

General references:

Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30(5):209-17.
PubMed

Product specific references:

Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M: Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004 Dec 1;173(11):6703-11.
PubMed
Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M: Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells. Eur J Immunol. 2010 Mar;40(3):722-32.
PubMed
Variant
0.1 mg
12-728-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Contact distributor
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