Anti-Hu CD300e APC

Anti-Hu CD300e APC
Regulatory status
RUO
Antigen
CD300e
Clone
UP-H2
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-728-T100
In stock
242.00 USD

25 tests
1A-728-T025
In stock
121.00 USD
Variant
100 tests
1A-728-T100
In stock
242.00 USD

25 tests
1A-728-T025
In stock
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
CD300e-HA-transfected cells
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2
Antigen description
CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.
Entrez Gene ID 342510
UniProt ID Q496F6
1A-728 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD300e (UP-H2) APC.

General references:

Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30(5):209-17.
PubMed

Product specific references:

Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M: Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004 Dec 1;173(11):6703-11.
PubMed
Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M: Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells. Eur J Immunol. 2010 Mar;40(3):722-32.
PubMed
Variant
100 tests
1A-728-T100
In stock
242.00 USD

25 tests
1A-728-T025
In stock
121.00 USD
Variant
100 tests
1A-728-T100
In stock
242.00 USD

25 tests
1A-728-T025
In stock
121.00 USD

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