Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD300e APC

Anti-Hu CD300e APC

Regulatory status

RUO

Antigen

CD300e

Clone

UP-H2

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-728-T100

In stock

220.00 USD

25 tests

1A-728-T025

Delivery 1 week

110.00 USD

Variant

100 tests

1A-728-T100

In stock

220.00 USD

25 tests

1A-728-T025

Delivery 1 week

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody UP-H2 recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD300e-HA-transfected cells

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLM2, CMRF35-A5, LMIR6, IREM-2, PlgR2

Antigen description

CD300e / IREM-2 (immune receptor expressed by myeloid cells 2), also known as CLM2 or LMIR6, is a monomeric transmembrane glycoprotein with a single extracellular immunoglobulin-like domain. Intracellularly it associates with DAP-12, an ITAM-containing adaptor molecule. CD300e is expressed on mature monocytes and peripheral blood myeloid dendritic cells. Its crosslinking leads to release of pro-inflammatory cytokines, and increased expression of activation markers.

Entrez Gene ID 342510

UniProt ID Q496F6

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD300e (UP-H2) APC.

General references:

Clark GJ, Ju X, Tate C, Hart DN: The CD300 family of molecules are evolutionarily significant regulators of leukocyte functions. Trends Immunol. 2009 May;30(5):209-17.
PubMed

Product specific references:

Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M: Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004 Dec 1;173(11):6703-11.
PubMed

Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M: Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells. Eur J Immunol. 2010 Mar;40(3):722-32.
PubMed