Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

Anti-Hu CD3 zeta (pY72) Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
CD3 zeta (pY72)
Clone
EM-26
Format
Alexa Fluor® 647
Reactivity
Mouse, Human
Application
FC (QC tested), ICC
Excitation laser
red (633 nm)
Variant
0.1 mg
A6-712-C100
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Product details
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Application
FC (QC tested), ICC
Reactivity
Mouse, Human
Immunogen
A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
Other names
CD3 zeta chain, CD247, T3Z, TCRz, IMD25
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A6-712_FC_Histogram
Separation of CD3 positive T cells stained using anti-human CD3 zeta (pY72) Alexa Fluor® 647 antibody (concentration in sample 3 μg/ml, red-filled) from T cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 3 μg/ml, black-dashed) in flow cytometry analysis (intracellular staining) of human PHA activated peripheral blood mononuclear cells.

Product specific references:

Dopfer EP, Schöpf B, Louis-Dit-Sully C, Dengler E, Höhne K, Klescová A, Prouza M, Suchanek M, Reth M, Schamel WW: Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling. Immunol Lett. 2010 May 4;130(1-2):43-50
PubMed
Borroto A, Arellano I, Dopfer EP, Prouza M, Suchànek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1;190(3):1103-12.
PubMed
Variant
0.1 mg
A6-712-C100
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD

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