Anti-Hu CD3 zeta (pY153) Purified

Western blotting analysis using monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) reacting with particular phosphorylated human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.

Anti-Hu CD3 zeta (pY153) purified antibody (clone EM-17) works in Flow Cytometry application

Analysis of the antibody staining was performed on Jurkat cells treated or untreated with pervanadate (PV) prior to the fixation and permeabilization of cell suspension with cold methanol.
Anti-Hu CD3 zeta (pY153) purified antibody (concentration in sample 1 μg/ml, GAM APC, red-filled histogram) binds specifically to phosphorylated tyrosine 153 (pY153) of CD3 zeta chain in PV treated, methanol permeabilized Jurkat cells (upper panel), but not to untreated methanol permeabilized control cells (lower panel).
Level of non-specific binding was assessed using Mouse IgG1 isotype control purified (MOPC-21) under same conditions (concentration in sample 1 μg/ml, GAM APC, black-dashed histogram).

Anti-Hu CD3 zeta (pY153) Purified (clone EM-17) specificity verification by WB.

The specificity of EM-17 antibody to phosphorylated Tyr 153 (CD3 zeta chain) was assessed by analysis of binding signals in HEK293T transfected with CD3 zeta/ZAP-70 construct followed by pervanadate (PV) treatment in comparison to the series of control cells – PV untreated transfectants, and both PV treated and untreated mock HEK293T cells.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer with PhosSTOP and pervanadate), mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody EM-17 (1 µg/ml). Subclass-specific secondary antibody IRDye 680LT Goat-anti-Mouse IgG (red) was used for fluorescent Western blot detection.