Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD3 zeta (pY153) Purified

Anti-Hu CD3 zeta (pY153) Purified

Regulatory status

RUO

Antigen

CD3 zeta (pY153)

Clone

EM-17

Format

Purified

Reactivity

Human, Mouse

Application

WB, FC (QC tested)

Variant

0.1 mg

11-686-C100

In stock

130.00 USD

0.025 mg

11-686-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-686-C100

In stock

130.00 USD

0.025 mg

11-686-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody EM-17 recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Application

WB, FC (QC tested)

Application details

Western blotting: Recommended dilution: 2 - 5 μg/ml; positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining; recommended dilution: 1-4 μg/ml; positive control: Jurkat cells treated with pervanadate.

Reactivity

Human, Mouse

Immunogen

A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CD3 zeta chain, CD247, T3Z, TCRz, IMD25

Antigen description

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Entrez Gene ID 919

UniProt ID P20963

Western blotting analysis using monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) reacting with particular phosphorylated human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.

Anti-Hu CD3 zeta (pY153) purified antibody (clone EM-17) specificity verification by flow cytometry

Anti-Hu CD3 zeta (pY153) purified antibody (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intracellular CD3 zeta (pY153) in pervanadate treated and then methanol permeabilized Jurkat cells (upper panel), but not to untreated methanol permeabilized Jurkat cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).