Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

Anti-Hu CD3 zeta (pY142) Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
CD3 zeta (pY142)
Clone
EM-54
Format
Alexa Fluor® 488
Reactivity
Human, Mouse
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
A4-730-C100
In stock
242.00 USD
Variant
0.1 mg
A4-730-C100
In stock
242.00 USD
Product details
Description
Images
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody EM-54 recognizes phosphorylated intracellular tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Application
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Reactivity
Human, Mouse
Immunogen
CP1 (pTyr142) - KLH
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
CD3 zeta chain, CD247, T3Z, TCRz, IMD25
Antigen description
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Entrez Gene ID 919
UniProt ID P20963
A4-730_FC_Profil Značení
Flow cytometry intracellular staining pattern of human stimulated (CD3 + CD28 + pervanadate)peripheral blood mononuclear cells stained using anti-human CD3 zeta (pY142) (EM-54) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml).
A4-730_FC_Dot-plot
Left: Flow cytometry multicolor intracellular staining of human nonstimulated CD3 positive lymphocytes stained using anti-human CD3 zeta (pY142) (EM-54) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml) and anti-human CD4 (MEM-241) APC antibody (4 μl reagent / 100 μl of human stimulated (CD3 + CD28 + pervanadate) peripheral blood mononuclear cells).
Left: Flow cytometry multicolor intracellular staining of human stimulated (CD3 + CD28 + pervanadate) CD3 positive lymphocytes stained using anti-human CD3 zeta (pY142) (EM-54) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml) and anti-human CD4 (MEM-241) APC antibody (4 μl reagent / 100 μl of Ficoll purified peripheral blood nucleated cells).
A4-730_FC_Dot-plot_2
Left: Flow cytometry multicolor intracellular staining of human nonstimulated CD3 positive lymphocytes stained using anti-human CD3 zeta (pY142) (EM-54) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml) and anti-human CD8 (MEM-31) Pacific Blue™ antibody (4 μl reagent / 100 μl of human stimulated (CD3 + CD28 + pervanadate) peripheral blood mononuclear cells).
Left: Flow cytometry multicolor intracellular staining of human stimulated (CD3 + CD28 + pervanadate) CD3 positive lymphocytes stained using anti-human CD3 zeta (pY142) (EM-54) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml) and anti-human CD8 (MEM-31) Pacific Blue™ antibody (4 μl reagent / 100 μl of Ficoll purified peripheral blood nucleated cells).
Variant
0.1 mg
A4-730-C100
In stock
242.00 USD
Variant
0.1 mg
A4-730-C100
In stock
242.00 USD

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