Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD3 zeta (pY111) Purified

Anti-Hu CD3 zeta (pY111) Purified

Regulatory status

RUO

Antigen

CD3 zeta (pY111)

Clone

EM-55

Format

Purified

Reactivity

Mouse, Human

Application

WB, FC (QC tested)

Variant

0.1 mg

11-737-C100

In stock

130.00 USD

0.025 mg

11-737-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-737-C100

In stock

130.00 USD

0.025 mg

11-737-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody EM-55 recognizes phosphorylated intracellular tyrosine 111 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Mouse, Human

Immunogen

BP1 (pTyr111/123) - KLH

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CD3 zeta chain, CD247, T3Z, TCRz, IMD25

Antigen description

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Entrez Gene ID 919

UniProt ID P20963

Western blotting analysis of cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines. The lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 (thus only pY111 remains native) 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 8: mut. mCD3zeta Y/F1-5

Variant

0.1 mg

11-737-C100

In stock

130.00 USD

0.025 mg

11-737-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-737-C100