Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD3 Purified Low Endotoxin

Anti-Hu CD3 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD3

Clone

MEM-92

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, FC (QC tested), FUNC

Variant

0.1 mg

12-381-C100

In stock

140.00 USD

Variant

0.1 mg

11-261-C100

In stock

140.00 USD

Contact distributor

Product details

Description

References

Isotype

Mouse IgM

Specificity

The antibody MEM-92 reacts with an extracellular epitope on epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.

Workshop

HLDA IV: WS Code T 97

Application

IP, FC (QC tested), FUNC

Application details

Functional application: The antibody MEM-92 in solution induces early responses of T cell activation (tyrosine phosphorylation, calcium elevation, Erk activation and expression of activation antigens), but it is unable to induce T cell proliferation.
Flow cytometry: Recommended dilution: 3-10 μg/ml.

Reactivity

Human

Immunogen

Human peripheral blood lymphocytes.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CD3E, T3E, TCRE

Antigen description

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Entrez Gene ID 916

UniProt ID P07766

General references:

Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30.
PubMed

Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5.
PubMed

Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
PubMed

Product specific references:

Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7.
PubMed

Brdicka T, Imrich M, Angelisová P, Brdicková N, Horváth O, Spicka J, Hilgert I, Lusková P, Dráber P, Novák P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsí V: Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16;196(12):1617-26.
PubMed

Stefanová I, Saville MW, Peters C, Cleghorn FR, Schwartz D, Venzon DJ, Weinhold KJ, Jack N, Bartholomew C, Blattner WA, Yarchoan R, Bolen JB, Horak ID: HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression. J Clin Invest. 1996 Sep 15;98(6):1290-7.
PubMed

Batista A, Millán J, Mittelbrunn M, Sánchez-Madrid F, Alonso MA: Recruitment of transferrin receptor to immunological synapse in response to TCR engagement. J Immunol. 2004 Jun 1;172(11):6709-14.
PubMed

Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
PubMed

Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71.
PubMed

Souček J, Hilgert I, Budová I, Lindnerová G: Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2. Neoplasma. 1994;41(2):75-81.
PubMed

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Variant

0.1 mg

12-381-C100

In stock

140.00 USD

Variant

0.1 mg

11-261-C100

In stock

140.00 USD

Contact distributor