Anti-Hu CD3 Purified

Anti-Hu CD3 Purified
Regulatory status
RUO
Antigen
CD3
Clone
TB3
Format
Purified
Reactivity
Human
Application
FC (QC tested), ICC
Variant
0.1 mg
11-815-C100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody TB3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, although not presented to HLDA workshops, has superior binding than the workshop clone TB2.
Workshop
HLDA 10, HLDA 11
Application
FC (QC tested), ICC
Reactivity
Human
Other names
CD3E, T3E, TCRE
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-815 FC
Flow cytometry analysis (surface staining) of human buffy coat cells with anti-CD3 (TB3) purified / GAM-APC.
11-815_ICC
Immunocytochemistry staining of human CD3 epsilon and CD3 gamma transfected COS cells using mouse monoclonal antibodies anti-CD3, clones UCHT1 (left) and TB3 (right) indicated by Alexa Fluor 555 signal (red); DNA stained by DAPI (blue).

General references:

Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30.
PubMed
Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5.
PubMed
Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
PubMed

Product specific references:

Khvastunova AN, Kuznetsova SA, Al-Radi LS, Vylegzhanina AV, Zakirova AO, Fedyanina OS, Filatov AV, Vorobjev IA, Ataullakhanov F: Anti-CD antibody microarray for human leukocyte morphology examination allows analyzing rare cell populations and suggesting preliminary diagnosis in leukemia. Sci Rep. 2015 Jul 27;5:12573.
PubMed
Variant
0.1 mg
11-815-C100
In stock
Variant
0.1 mg
11-261-C100
In stock