Anti-Hu CD3 PE-DyLight® 594

Anti-Hu CD3 PE-DyLight® 594
Regulatory status
RUO
Antigen
CD3
Clone
MEM-57
Format
PE-DyLight® 594
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T5-202-T100
In stock
250.00 USD

25 tests
T5-202-T025
Delivery 1 week
125.00 USD
Variant
0.1 mg
11-261-C100
In stock
250.00 USD

0.1 mg
11-261-C100
In stock
125.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The antibody MEM-57 reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
Workshop
HLDA IV: WS Code T 96
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human thymocytes and T lymphocytes.
Preparation
The purified antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Other names
CD3E, T3E, TCRE
Antigen description
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Entrez Gene ID 916
UniProt ID P07766
T5-202_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (MEM-57) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T5-202_FC_Histogram
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (MEM-57) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30.
PubMed
Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5.
PubMed
Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
PubMed

Product specific references:

Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.
PubMed
Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95.
PubMed
Horejsi V. et al.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol. (Praha) 34, 23 (1988).
PubMed
Hilgert I. et al.: Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55, 435 (1993).
PubMed
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989); p. 293.
Dave VP, Cao Z, Browne C, Alarcon B, Fernandez-Miguel G, Lafaille J, de la Hera A, Tonegawa S, Kappes DJ: CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage. EMBO J. 1997 Mar 17;16(6):1360-70.
PubMed
Panyi G, Bagdány M, Bodnár A, Vámosi G, Szentesi G, Jenei A, Mátyus L, Varga S, Waldmann TA, Gáspar R, Damjanovich S: Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2592-7.
PubMed
Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
PubMed
Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
PubMed
Soucek J, Chudomel V, Hrubá A, Lindnerová G: Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3. Neoplasma. 1991;38(1):33-41.
PubMed
Soucek J, Hilgert I, Budová I, Lindnerová G: Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2. Neoplasma. 1994;41(2):75-81.
PubMed
Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611.
Variant
100 tests
T5-202-T100
In stock
250.00 USD

25 tests
T5-202-T025
Delivery 1 week
125.00 USD
Variant
0.1 mg
11-261-C100
In stock
250.00 USD

0.1 mg
11-261-C100
In stock
125.00 USD