Anti-Hu CD3 APC-Cy™7

Anti-Hu CD3 APC-Cy™7
Regulatory status
RUO
Antigen
CD3
Clone
SK7
Format
APC-Cy™7
Reactivity
Non-human primates, Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
T4-173-T100
Ask for delivery term HERE
275.00 USD

25 tests
T4-173-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T4-173-T100
Ask for delivery term HERE
275.00 USD

25 tests
T4-173-T025
Delivery 1 week
137.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody SK7 recognizes an extracellular epitope on the CD3 antigen on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.
Workshop
HLDA II: WS Code T118; HLDA III: WS Code T492
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
Reactivity
Non-human primates, Human
Immunogen
Human thymocytes
Preparation
Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
CD3E, T3E, TCRE
Antigen description
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Entrez Gene ID 916
UniProt ID P07766
T4-173_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (SK7) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T4-173_FC_Histogram
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30.
PubMed
Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5.
PubMed
Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
PubMed

Product specific references:

Qiao D, Li L, Guo J, Lao S, Zhang X, Zhang J, Wu C: Mycobacterium tuberculosis culture filtrate protein 10-specific effector/memory CD4⁺ and CD8⁺ T cells in tubercular pleural fluid, with biased usage of T cell receptor Vβ chains. Infect Immun. 2011 Aug;79(8):3358-65.
PubMed
Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120(3):371-9.
PubMed
Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005 Dec;116(4):487-98.
PubMed
Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Høybjør S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J Immunol. 2007 Feb 15;178(4):2556-64.
PubMed
Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T-cell hyporesponsiveness. J Invest Dermatol. 2008 May;128(5):1182-91.
PubMed
Fromm JR, Thomas A, Wood BL: Characterization and purification of neoplastic cells of nodular lymphocyte predominant hodgkin lymphoma from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Pathol. 2017 Feb;187(2):304-317.
PubMed
van Dongen JJ, Krissansen GW, Wolvers-Tettero IL, Comans-Bitter WM, Adriaansen HJ, Hooijkaas H, van Wering ER, Terhorst C: Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood 1988 Mar;71(3):603-12.
PubMed
Variant
100 tests
T4-173-T100
Ask for delivery term HERE
275.00 USD

25 tests
T4-173-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T4-173-T100
Ask for delivery term HERE
275.00 USD

25 tests
T4-173-T025
Delivery 1 week
137.50 USD

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