Anti-Hu CD3 APC

Anti-Hu CD3 APC
Regulatory status
RUO
Antigen
CD3
Clone
TB3
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-815-T100
Ask for delivery term HERE
242.00 USD
Variant
100 tests
1A-815-T100
Ask for delivery term HERE
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody TB3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody has superior binding than the clone TB2.
Workshop
HLDA XI
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CD3E, T3E, TCRE
Antigen description
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Entrez Gene ID 916
UniProt ID P07766
1A-815_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (TB3) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-815_FC_Histogram
Separation of human CD3 positive lymphocytes (red-filled) from CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (TB3) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30.
PubMed
Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5.
PubMed
Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
PubMed

Product specific references:

Khvastunova AN, Kuznetsova SA, Al-Radi LS, Vylegzhanina AV, Zakirova AO, Fedyanina OS, Filatov AV, Vorobjev IA, Ataullakhanov F: Anti-CD antibody microarray for human leukocyte morphology examination allows analyzing rare cell populations and suggesting preliminary diagnosis in leukemia. Sci Rep. 2015 Jul 27;5:12573.
PubMed
Variant
100 tests
1A-815-T100
Ask for delivery term HERE
242.00 USD
Variant
100 tests
1A-815-T100
Ask for delivery term HERE
242.00 USD

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