Anti-Hu CD294 Purified

Anti-Hu CD294 Purified
Regulatory status
RUO
Antigen
CD294
Clone
BM16
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-144-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Rat IgG2a
Specificity
The rat monoclonal antibody BM16 recognizes an extracellular epitope of CD294 / CRTH2 (prostaglandin D2 receptor 2), a G-protein-coupled seven-transmembrane protein expressed on Th2 cells, peripheral blood basophils and eosinophils.
Workshop
HLDA VIII
Application
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Reactivity
Human
Immunogen
Human CD294 transfected rat cell line TART/B19-12.10
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
DP2, PTGDR2, DL1R, CRTH2, GPR44
Antigen description
CD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.
Entrez Gene ID 11251
UniProt ID Q9Y5Y4
11-144_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD294/CRTH2 (BM16) purified antibody (concentration in sample 5,0 μg/ml, GAR APC) and anti-human CD4 (MEM-241) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
11-144_FC_Histogram
Separation of human CD294 positive lymphocytes (red-filled) from CD294 negative lymphocytes in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD294/CRTH2 (BM16) purified antibody (concentration in sample 5,0 μg/ml, GAR APC).

Product specific references:

De Fanis U, Mori F, Kurnat RJ, Lee WK, Bova M, Adkinson NF, Casolaro V: GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007 May 15;109(10):4343-50. Epub 2007 Jan 18.
PubMed
Mjösberg J, Bernink J, Golebski K, Karrich JJ, Peters CP, Blom B, te Velde AA, Fokkens WJ, van Drunen CM, Spits H: The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells. Immunity. 2012 Oct 19;37(4):649-59.
PubMed
Bellinghausen I, Reuter S, Martin H, Maxeiner J, Luxemburger U, Türeci Ö, Grabbe S, Taube C, Saloga J: Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity. J Allergy Clin Immunol. 2012 Dec;130(6):1384-93.
PubMed
Nagata K, Tanaka K, Ogawa K, Kemmotsu K, Imai T, Yoshie O, Abe H, Tada K, Nakamura M, Sugamura K, Takano S: Selective expression of a novel surface molecule by human Th2 cells in vivo. J Immunol. 1999 Feb 1;162(3):1278-86.
PubMed
Variant
0.1 mg
11-144-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

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