Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD28 Purified Low Endotoxin

Anti-Hu CD28 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD28

Clone

CD28.2

Format

Purified Low Endotoxin

Reactivity

Non-human primates, Human

Application

IP, WB, FC (QC tested), IHC-F, ICC, FUNC

Variant

0.1 mg

12-577-C100

In stock

147.40 USD

Variant

0.1 mg

12-577-C100

In stock

147.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Workshop

HLDA V: WS Code 5T CD28.05

Application

IP, WB, FC (QC tested), IHC-F, ICC, FUNC

Application details

Functional application: T cell costimulation.
Flow cytometry: Recommended dilution: 1-12 µg/ml

Reactivity

Non-human primates, Human

Immunogen

DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TP44

Antigen description

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Entrez Gene ID 940

UniProt ID P10747

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (low endotoxin, concentration in sample 1,6 μg/ml) GAM APC.

Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (low endotoxin, concentration in sample 1,6 μg/ml) GAM APC.

Product specific references:

Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001 Jan 1;166(1):197-206.
PubMed

Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004 May;24(9):3860-73.
PubMed

Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004 Jul;78(13):6995-7003.
PubMed

Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993 Mar;5(3):311-5.
PubMed

Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J. 1993 Aug 1;293 ( Pt 3):835-42.
PubMed

Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol. 1999 Jul 1;163(1):62-70.
PubMed

Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol. 1999 Sep 15;163(6):3511-5.
PubMed

Mary C, Coulon F, Poirier N, Dilek N, Martinet B, Blancho G, Vanhove B: Antagonist properties of monoclonal antibodies targeting human CD28: Role of valency and the heavy-chain constant domain. MAbs Jan-Feb 2013;5(1):47-55.
PubMed