Anti-Hu CD28 PE-Cy™7

Anti-Hu CD28 PE-Cy™7
Regulatory status
RUO
Antigen
CD28
Clone
CD28.2
Format
PE-Cy™7
Reactivity
Non-human primates, Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T7-577-T100
In stock
275.00 USD

25 tests
T7-577-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T7-577-T100
In stock
275.00 USD

25 tests
T7-577-T025
Delivery 1 week
137.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody CD28.2 recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Workshop
HLDA V: WS Code 5T CD28.05
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
TP44
Antigen description
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Entrez Gene ID 940
UniProt ID P10747
T7-577 FC
Flow cytometry analysis (surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) PE-Cy™7.

Product specific references:

Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001 Jan 1;166(1):197-206.
PubMed
Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004 May;24(9):3860-73.
PubMed
Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004 Jul;78(13):6995-7003.
PubMed
Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993 Mar;5(3):311-5.
PubMed
Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J. 1993 Aug 1;293 ( Pt 3):835-42.
PubMed
Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol. 1999 Jul 1;163(1):62-70.
PubMed
Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol. 1999 Sep 15;163(6):3511-5.
PubMed
Mary C, Coulon F, Poirier N, Dilek N, Martinet B, Blancho G, Vanhove B: Antagonist properties of monoclonal antibodies targeting human CD28: Role of valency and the heavy-chain constant domain. MAbs Jan-Feb 2013;5(1):47-55.
PubMed
Variant
100 tests
T7-577-T100
In stock
275.00 USD

25 tests
T7-577-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T7-577-T100
In stock
275.00 USD

25 tests
T7-577-T025
Delivery 1 week
137.50 USD

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